ArticleBMC veterinary research2026
Biological effects of orotic acid on ovine mammary epithelial cells and associated regulatory pathways study by transcriptomics.
Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Bioactive Substances in Sheep's Milk and Related Products as Components of Functional Foods.Molecules (Basel, Switzerland) · 2026Review
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10 authors.
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Abstract
backgroundOrotic acid (OA) is known to promote cell proliferation and influence triglyceride (TG) and protein metabolism in several animal species and cell types. However, its biological function in ovine mammary epithelial cells (OMECs) remains unclear. In this study, we evaluated the effects of OA on cell proliferation, oxidative stress, and synthesis of lactose, milk fat, and protein, and the explored potential regulatory mechanism using transcriptomic analysis.
resultsTreatment with 30 µg/mL of OA significantly promoted OMEC proliferation. OA also improved oxidative stress related indicators, increasing CAT (14.71 ± 3.27 VS 6.90 ± 1.40 U/106cell, P < 0.05) and SOD (7.08 ± 0.86 VS 4.18 ± 1.32 U/106cell, P < 0.05) activates, and reducing MDA (38.55 ± 12.06 VS 80.19 ± 12.34 U/106cell, P < 0.05). TG concentrations increased significantly both in cells (12.58 ± 1.86 VS 2.69 ± 0.23 µg/106 cells, P < 0.05) and culture supernatant (8.54 ± 0.62 VS 2.82 ± 0.12 ng/mL, P < 0.001). In contrast, OA significantly decreased the CSN2 concentration in the supernatant (24.41 ± 0.98 VS 34.62 ± 0.59 ng/mL, P < 0.001). Transcriptomic analysis identified 365 differentially expressed genes (DEGs), including 94 up-regulated and 271 down-regulated genes in the OA treated group. Up-regulated DEGs were enriched in the JAK-STAT signaling pathway and so on, while down-regulated DEGs were enriched in the protein digestion and absorption and so on. DEGs such as RPS6KA2 and FOXO4 were associated with cell proliferation, and ALDOC, SULT2B1, ADRB1, STAT5A were associated with fat metabolism.
conclusionThese findings suggest that OA enhances cells proliferation, improves oxidative stress indicators, and promotes TG synthesis in OMECs. The study provides a useful reference for understanding the regulation mechanism of lactation traits and for dairy sheep breeding.
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