Evidence map›Paper›PMID 42000972›Full record

ArticleHistochemistry and cell biology2026

Fiber type-specific expression of LACTB leverages a function in oxidative metabolism.

Kirsi-Marja Alanen, Rabah Soliymani, Jaakko Sarparanta, Satu Kuure, Kirsi Sainio, Zydrune Polianskyte, Muhammad Yasir Asghar, Ehsan Zangene, Annunziata Cascone, Maciej Lalowski and 4 more

Abstract read
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In one paragraph

Article in Histochemistry and cell biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. April In focus in HCB: skeletal muscle fibers.Histochemistry and cell biology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Kirsi-Marja AlanenBiochemistry and Developmental Biology, Medicum, Faculty of Medicine, University of Helsinki, Biomedicum Helsinki 1, Haartmaninkatu 8, 00290, Helsinki, Finland.
Rabah SoliymaniMeilahti Proteomics Unit, Faculty of Medicine, HiLIFE, University of Helsinki, Helsinki, Finland.
Jaakko SarparantaFolkhälsan Research Center, Helsinki, Finland.
Satu KuureGM-Unit, HiLIFE, University of Helsinki, Helsinki, Finland.
Kirsi SainioBiochemistry and Developmental Biology, Medicum, Faculty of Medicine, University of Helsinki, Biomedicum Helsinki 1, Haartmaninkatu 8, 00290, Helsinki, Finland.
Zydrune PolianskyteBiochemistry and Developmental Biology, Medicum, Faculty of Medicine, University of Helsinki, Biomedicum Helsinki 1, Haartmaninkatu 8, 00290, Helsinki, Finland.
Muhammad Yasir AsgharBiochemistry and Developmental Biology, Medicum, Faculty of Medicine, University of Helsinki, Biomedicum Helsinki 1, Haartmaninkatu 8, 00290, Helsinki, Finland.
Ehsan ZangeneBiochemistry and Developmental Biology, Medicum, Faculty of Medicine, University of Helsinki, Biomedicum Helsinki 1, Haartmaninkatu 8, 00290, Helsinki, Finland.
Annunziata CasconeBiochemistry and Developmental Biology, Medicum, Faculty of Medicine, University of Helsinki, Biomedicum Helsinki 1, Haartmaninkatu 8, 00290, Helsinki, Finland.
Maciej LalowskiMeilahti Proteomics Unit, Faculty of Medicine, HiLIFE, University of Helsinki, Helsinki, Finland.
Peter HackmanFolkhälsan Research Center, Helsinki, Finland.
Johan LundinInstitute for Molecular Medicine Finland (FIMM), HiLIFE, University of Helsinki, Helsinki, Finland.
Dan LindholmBiochemistry and Developmental Biology, Medicum, Faculty of Medicine, University of Helsinki, Biomedicum Helsinki 1, Haartmaninkatu 8, 00290, Helsinki, Finland.
Ove ErikssonBiochemistry and Developmental Biology, Medicum, Faculty of Medicine, University of Helsinki, Biomedicum Helsinki 1, Haartmaninkatu 8, 00290, Helsinki, Finland. ove.eriksson@helsinki.fi.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Skeletal muscle is composed of type I and type II fibers, each characterized by specific metabolic machinery. LACTB is a conserved mitochondrial protein implicated in lipid utilization and tumorigenesis, but its precise function in the cellular metabolism remains unclear. To gain novel insight into the functional role of LACTB, we investigated skeletal muscle to determine whether LACTB is segregated by fiber type. The expression of LACTB was determined by immunohistochemistry (IHC) and immunoblotting in skeletal muscle from healthy human subjects and the laboratory rat. The specificity of the antibody was assessed using recombinant human LACTB protein and endogenous LACTB in isolated mitochondria. IHC results were validated in a cellular model of myoblast differentiation using the C2C12 and L6 cell lines. The results demonstrated that LACTB is highly enriched in adult type I muscle fibers. During development, LACTB expression commences in type I primary myotubes at the time of their formation around week 20 of gestational age. LACTB expression in myoblasts is low but increases rapidly upon the induction of myotube differentiation. We conclude that LACTB plays a distinct role in mitochondria of type I fibers, most likely acting in oxidative metabolism related to energy use from lipids. This defines LACTB as a mitochondrial marker for type I fibers.

Indexed as

Mitochondrial ProteinsMuscle Fibers, SkeletalAnimalsHumansMiceOxidation-ReductionRatsMitochondrial ProteinsLACTBMitochondriaMuscle differentiationSerine proteaseType II muscle fiberType I muscle fiber

Identifiers

PMID42000972
PMCPMC13091871

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.