Evidence map›Paper›PMID 41999982›Full record

ArticleJournal of lipid research2026

Hydrogen gas with hypothermic machine perfusion induces lipidomic alterations in donation-after-cardiac-death rat livers.

Yusuke Minami, Siddabasave Gowda B B Gowda, Kengo Shibata, Sodai Sakamoto, Divyavani Gowda, Tsuyoshi Shimamura, Akinobu Taketomi, Hitoshi Chiba, Moto Fukai, Shu-Ping Hui

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Article in Journal of lipid research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yusuke MinamiGraduate School of Health Sciences, Hokkaido University, Kita-ku, Sapporo, Japan.
Siddabasave Gowda B B GowdaFaculty of Health Sciences, Hokkaido University, Kita-ku, Sapporo, Japan; Graduate School of Global Food Resources, Hokkaido University, Kita-Ku, Sapporo, Japan.
Kengo ShibataGraduate School of Medicine, Gastroenterological Surgery 1, Hokkaido University, Kita-ku, Sapporo, Japan.
Sodai SakamotoGraduate School of Medicine, Gastroenterological Surgery 1, Hokkaido University, Kita-ku, Sapporo, Japan.
Divyavani GowdaFaculty of Health Sciences, Hokkaido University, Kita-ku, Sapporo, Japan.
Tsuyoshi ShimamuraDivision of Organ Transplantation, Hokkaido University Hospital, Kita-ku, Sapporo, Japan.
Akinobu TaketomiGraduate School of Medicine, Gastroenterological Surgery 1, Hokkaido University, Kita-ku, Sapporo, Japan.
Hitoshi ChibaDepartment of Nutrition, Sapporo University of Health Sciences, Higashi-ku, Sapporo, Japan.
Moto FukaiGraduate School of Medicine, Gastroenterological Surgery 1, Hokkaido University, Kita-ku, Sapporo, Japan; Graduate School of Well-being, Department of Food Environment, Fuji Women's University, Ishikari, Japan. Electronic address: db7mfki@gmail.com.
Shu-Ping HuiFaculty of Health Sciences, Hokkaido University, Kita-ku, Sapporo, Japan. Electronic address: keino@hs.hokudai.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hypothermic machine perfusion (HMP) combined with hydrogen gas has previously been shown to mitigate ischemia-reperfusion injury (IRI) in rat liver, but without full recovery of function. This study examined how hydrogen gas modulates hepatic lipid metabolism during HMP using donation after cardiac death (DCD) rat livers. Untargeted liquid chromatography-mass spectrometry was employed to perform comprehensive lipidomic profiling of liver samples. The analysis results revealed distinct lipid metabolic alterations across cold storage, machine perfusion (MP), and hydrogen-supplemented perfusion (MP-H2) groups compared to healthy controls. Compared with MP, MP-H2 treatment reduced lysophosphatidylinositol (LPI) levels and the LPI/phosphatidylinositol ratio while increasing phosphatidic acid (PA) species such as PA (18:0/18:1) and PA (18:0/20:4). Elevated lysophosphatidylethanolamine and ceramide in MP-H2 suggested adaptive remodeling of membrane lipids. The ratio of monolysocardiolipin to cardiolipin increased in the MP group, but was reduced following hydrogen gas treatment. These lipidomic shifts imply that hydrogen gas attenuates IRI by stabilizing lipid homeostasis and may improve DCD graft viability. Furthermore, the restoration of key lipid species associated with mitochondrial integrity and membrane remodeling suggests that hydrogen gas supports bioenergetic recovery and limits oxidative membrane damage during reperfusion. Overall, these findings highlight the potential of hydrogen-enriched perfusion as a metabolic intervention to enhance organ preservation, reduce mitochondrial dysfunction, and extend the useable lifespan of DCD liver grafts for transplantation.

Indexed as

DeathHydrogenLipid MetabolismLipidomicsLiverPerfusionAnimalsMaleOrgan PreservationRatsHydrogenischemia reperfusion injurylipid metabolismlipidomicslysophospholipidmitochondriaphosphatidic acidphospholipids

Identifiers

PMID41999982
PMCPMC13279156

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.