Evidence map›Paper›PMID 41999294›Full record

ArticleJournal of insect science (Online)2026

A novel rapid detection approach based on CRISPR-Cas13a for Dermatophagoides pteronyssinus and Dermatophagoides farinae (Acariformes: Pyroglyphidae).

Siwen Liu, Lan Wang, Hong Zhang, Feng Jiang, Feifan Tang, Renren Han, Wei Guo, Shengli Gu, Guoqing Chen, Dacun Zhang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of insect science (Online), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Siwen LiuDepartment of Medical Parasitology, Wannan Medical University, Wuhu, Anhui, China.ORCID 0009-0003-8813-4061
Lan WangDepartment of Medical Parasitology, Wannan Medical University, Wuhu, Anhui, China.
Hong ZhangAuhui Provincial Engineering Research Centre for Molecular Detection and Diagnostics, Anhui Normal University, Wuhu, China.
Feng JiangDepartment of Medical Parasitology, Wannan Medical University, Wuhu, Anhui, China.
Feifan TangDepartment of Medical Parasitology, Wannan Medical University, Wuhu, Anhui, China.
Renren HanDepartment of Medical Parasitology, Wannan Medical University, Wuhu, Anhui, China.ORCID 0009-0009-5790-6261
Wei GuoDepartment of Medical Parasitology, Wannan Medical University, Wuhu, Anhui, China.
Shengli GuDepartment of Medical Parasitology, Wannan Medical University, Wuhu, Anhui, China.
Guoqing ChenShandong Jiuxin Bioengineering Co., Ltd, Shandong, China.
Dacun ZhangShandong Jiuxin Bioengineering Co., Ltd, Shandong, China.
Xiaodong ZhanDepartment of Medical Parasitology, Wannan Medical University, Wuhu, Anhui, China.ORCID 0000-0002-7224-5248

Funding

Commissioned Research Project and the Anhui Provincial Graduate Innovation and Entrepreneurship Practice Project 2022cxcysj180Commissioned Research Project and the Anhui Provincial Graduate Innovation and Entrepreneurship Practice Project H202112
6 · The paper itself

Abstract

Dermatophagoides pteronyssinus (Trouessart) and Dermatophagoides farinae (Hughes) (Acariformes: Pyroglyphidae) are the prevalent kinds of house dust mites (HDMs). HDM is a common indoor pest, which mainly breeds in indoor dust and is an important allergen source causing a variety of allergic diseases. Effective detection of these HDMs is crucial in preventing the allergic diseases they cause. The objective of this study was to develop an innovative method for the rapid visualization of HDMs (D. pteronyssinus and D. farinae) using recombinase polymerase amplification (RPA) and lateral flow dipstick (LFD) in combination with CRISPR-Cas13a (RPA-Cas13a-LFD). To achieve heightened sensitivity in the detection of HDMs, Cas13a was incorporated into the RPA process and coupled with T7 transcripts. Based on this approach, a total of 2.23-102 copies/μl of HDM were detected within 35 min (detection limit of 2.23 copies/μl for D. farinae and 39.7 copies/μl for D. pteronyssinus). No cross-reactivity occurred with Aleuroglyphus ovatus (Troupeau) (Acariformes: Acaridae), Tyrophagus putrescentiae (Schrank) (Acariformes: Acaridae), Blomia tropicalis (van Bronswijk, de Cock & Oshima) (Acariformes: Acaridae), Suidasia nesbitti (Hughes) (Acariformes: Acaridae), and Carpoglyphus lactis(Linnaeus) (Acariformes: Acaridae). The RPA-Cas13a-LFD methodology demonstrated high specificity and sensitivity in detecting HDM. Given its advantages, such as ease of operation, rapid detection, and time efficiency, it is well-suited for rapid field-based detection of HDMs, providing a new technical tool for detecting D. farinae and D. pteronyssinus.

Indexed as

CRISPR-Cas SystemsDermatophagoides farinaeDermatophagoides pteronyssinusAnimalsRapid Diagnostic TestsSensitivity and SpecificityAllergiesEnvironmental allergen testingMolecular diagnostics

Identifiers

PMID41999294
PMCPMC13091095

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.