ArticleJournal of the science of food and agriculture2026
Insect-resistant soybean genotypes accumulate rutin and its O-methylated derivative narcissin, a more potent flavonol targeting Anticarsia gemmatalis digestive and detoxification enzymes and reducing larval survival.
Jessica Nunes de Assis, Valquira Joana Medina Pinheiro, Jodieh Oliveira Santana Varejão, Ian de Paula Alves Pinto, André Luiz Lourenção, Nathália Silva Oliveira, Giovanna Cassia Mendes Moraes, Eduardo Vinícius Vieira Varejão, Maria Goreti de Almeida Oliveira, Humberto Josué de Oliveira Ramos
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In one paragraphArticle in Journal of the science of food and agriculture, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
10 authors.
Jessica Nunes de AssisLaboratory of Enzymology and Biochemistry of Proteins and Peptides, Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, UFV, BIOAGRO/INCT-IPP, Viçosa, Brazil.
Valquira Joana Medina PinheiroLaboratory of Enzymology and Biochemistry of Proteins and Peptides, Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, UFV, BIOAGRO/INCT-IPP, Viçosa, Brazil.
Jodieh Oliveira Santana VarejãoDepartamento de Química, Universidade Federal de Viçosa, Viçosa, Brazil.
Ian de Paula Alves PintoLaboratory of Enzymology and Biochemistry of Proteins and Peptides, Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, UFV, BIOAGRO/INCT-IPP, Viçosa, Brazil.
André Luiz LourençãoEscola Superior de Agricultura "Luiz de Queiroz" - USP, Piracicaba, Brazil.
Nathália Silva OliveiraLaboratory of Enzymology and Biochemistry of Proteins and Peptides, Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, UFV, BIOAGRO/INCT-IPP, Viçosa, Brazil.
Giovanna Cassia Mendes MoraesLaboratory of Enzymology and Biochemistry of Proteins and Peptides, Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, UFV, BIOAGRO/INCT-IPP, Viçosa, Brazil.
Maria Goreti de Almeida OliveiraLaboratory of Enzymology and Biochemistry of Proteins and Peptides, Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, UFV, BIOAGRO/INCT-IPP, Viçosa, Brazil.ORCID https://orcid.org/0000-0003-4862-7657 Humberto Josué de Oliveira RamosLaboratory of Enzymology and Biochemistry of Proteins and Peptides, Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, UFV, BIOAGRO/INCT-IPP, Viçosa, Brazil.ORCID https://orcid.org/0000-0001-6447-8108 Funding
National Institute of Science and Technology in Plant-Pest Interaction (INCT-IPP), Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) APQ-01338-23National Institute of Science and Technology in Plant-Pest Interaction (INCT-IPP), Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) APQ-03986-24National Institute of Science and Technology in Plant-Pest Interaction (INCT-IPP), Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) CNPq-403 431/2025-5National Institute of Science and Technology in Plant-Pest Interaction (INCT-IPP), Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) FAPEMIG: APQ-05406-24
6 · The paper itselfAbstract
backgroundSoybean underpins Brazil's agricultural, yet its sustainability is threatened by lepidopteran pests such as Anticarsia gemmatalis. Although insect-resistant cultivars have long been associated with the constitutive accumulation of quercetin-derived flavonols, the biochemical mechanisms underlying this resistance remain poorly understood. In particular, the role of flavonol O-methylation in enhancing plant defense has not been demonstrated.
resultsHere we combined metabolite identification, biological assays, and structure-based modeling to investigate flavonol-mediated resistance in soybean. The resistant genotype IAC 17 was found to accumulate not only rutin (quercetin 3-O-rutinoside; [M + H]
conclusionThese findings support a dual-ligand model in which rutin and narcissin cooperatively disrupt digestive and detoxification pathways in the insect midgut. The discovery of a constitutive flavonol O-methylation branch identifies narcissin as a potent defensive metabolite and provides mechanistic targets for breeding and metabolic engineering strategies aimed at developing soybean cultivars with improved resistance. © 2026 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Indexed as
FlavonolsGlycine maxMothsRutinAnimalsGenotypeInactivation, MetabolicLarvaMethylationMolecular Docking SimulationQuercetinFlavonolsQuercetinRutininsect pestLC‐MSmethylated flavonolmolecular dockingNMR
Identifiers
PMID41999131
PMCPMC13258211
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