Evidence map›Paper›PMID 41998717›Full record

ArticleJournal of ovarian research2026

BMP15 regulates proliferation of sheep granulosa cells with different genotypes via the TGF-β/SMAD signaling pathway.

Qing Liu, Guiling Cao, Wentao Li, Ziyi Liu, Peng Wang, Xiaoyun He, Yufang Liu, Mingxing Chu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of ovarian research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Qing Liu *State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Rd., Beijing, 100193, China.
Guiling Cao *College of Agriculture and Biology, Liaocheng University, Liaocheng, 25200, China.
Wentao LiState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Rd., Beijing, 100193, China.
Ziyi LiuState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Rd., Beijing, 100193, China.
Peng WangState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Rd., Beijing, 100193, China.
Xiaoyun HeState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Rd., Beijing, 100193, China.
Yufang LiuState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Rd., Beijing, 100193, China. aigaiy@126.com.
Mingxing ChuState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Rd., Beijing, 100193, China. mxchu@263.net.

Funding

Agricultural Science and Technology Innovation Program of China CAAS-ZDRW202502 and ASTIP-IAS13China Agriculture Research System of MOF and MARA CARS-38-02National Natural Science Foundation of China 32272838Postdoctoral Fellowship Program of CPSF GZC20233053Science and Technology Achievement Demonstration Project of Xinjiang Uygur Autonomous Region 2022NC116The Open Project of State Key Laboratory of Animal Biotech Breeding of China 2024SKLAB6-101
6 · The paper itself

Abstract

BMPR1B is considered to be the main effector gene affecting polytocous trait in sheep and other animals, and co-regulates reproductive performance with BMP15. Individuals containing the BMPR1B mutation had significantly higher lambing numbers than the wild type in Small-tailed Han sheep. However, the mode of regulation of BMP15 in the three genotypes (FecB BB, FecB + + and FecB + B) of BMPR1B in the Small-tailed Han sheep remains unclear. To investigate the effect of BMP15 on the proliferation of granulosa cells (GCs) from sheep with different FecB genotypes via the TGF-β/SMAD signaling pathway, we established an in vitro three-stage culture model (24h, 48h, 72h) using isolated GCs of three genotypes. We observed that BMP15 promoted GCs proliferation across all genotypes, with proliferative activity increasing progressively over the culture period. This BMP15-mediated proliferative response was associated with the specific upregulation of key components within the TGF-β/SMAD pathway. Notably, the FecB BB genotype exhibited the highest proliferation efficiency. Furthermore, compared to the FecB + + and FecB + B genotypes, the FecB BB genotype displayed the highest mRNA and protein expression levels of both TGFβ receptor I (TGFβ-R1) and SMAD Family Member 4 (SMAD4). In summary, it was found that BMP15 regulated GCs proliferation by affecting the expression of genes related to the TGF-β/SMAD signalling pathway. Compared with the FecB + + and FecB + B genotypes, the FecB BB genotype responded more strongly to BMP15 treatment, suggesting a genotype-dependent response. These results provided a basis for further investigation of the molecular mechanisms by which BMP15 regulates GCs proliferation.

Indexed as

Bone Morphogenetic Protein 15Granulosa CellsSmad ProteinsTransforming Growth Factor betaAnimalsBone Morphogenetic Protein Receptors, Type ICell ProliferationCells, CulturedFemaleGenotypeSheepSignal TransductionBone Morphogenetic Protein 15Bone Morphogenetic Protein Receptors, Type ISmad ProteinsTransforming Growth Factor betaBMP15Granulosa cellsSmall-tailed Han sheepTaqMan probesTGF-β signaling pathway

Identifiers

PMID41998717
PMCPMC13220390

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.