Evidence map›Paper›PMID 41998287›Full record

ArticleNature cell biology2026

A transgene-free, human peri-gastrulation embryo model presents trilaminar embryonic disc-, amnion- and yolk sac-like structures.

Shiyu Sun, Yi Zheng, Yung Su Kim, Zheng Zhong, Norio Kobayashi, Xufeng Xue, Yue Liu, Zhuowei Zhou, Yanhong Xu, Jinglei Zhai and 2 more

Abstract read
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In one paragraph

Article in Nature cell biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Shiyu SunDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA.
Yi ZhengDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA.ORCID http://orcid.org/0000-0002-2685-3680
Yung Su KimDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA.
Zheng ZhongDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA.
Norio KobayashiDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA.ORCID http://orcid.org/0009-0007-8718-6010
Xufeng XueDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA.ORCID http://orcid.org/0000-0002-9379-8589
Yue LiuDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA.
Zhuowei ZhouDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA.
Yanhong XuState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Jinglei ZhaiState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.ORCID http://orcid.org/0000-0002-9459-8781
Hongmei WangState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.ORCID http://orcid.org/0000-0003-1603-4785
Jianping FuDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA. jpfu@umich.edu.ORCID http://orcid.org/0000-0001-9629-6739

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human peri-gastrulation is a critical developmental stage, yet challenging to study directly. Stem cell-based embryo models have emerged as promising tools for probing early human embryogenesis. Here we report a transgene-free human embryo model, namely peri-gastrulation trilaminar embryonic disc (PTED) embryoid, derived exclusively from primed human pluripotent stem cells, recapitulating certain features of peri-gastrulation human development, which include the formation of trilaminar embryonic layers positioned between the dorsal amnion and ventral definitive yolk sac, as well as primitive haematopoiesis. Our lineage tracing showed that, in PTED embryoids, embryonic and extraembryonic mesoderm as well as embryonic and extraembryonic endoderm arise from gastrulating epiblast-like cells, which provides support for extraembryonic lineage potential of peri-gastrulation human epiblast. Notably, active haematopoiesis and blood-cell generation occurred within the definitive yolk sac-like structure of PTED embryoids. Together, PTED embryoids offer a tractable and ethically less complex model for investigating the self-organizing properties of human peri-gastrulation development.

Indexed as

AmnionEmbryoid BodiesGastrulationYolk SacAnimalsCell DifferentiationCell LineageEndodermGerm LayersHematopoiesisHumansMesodermPluripotent Stem CellsTransgenes

Identifiers

PMID41998287

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.