Evidence map›Paper›PMID 41997361›Full record

ArticleCellular and molecular gastroenterology and hepatology2026

Maternal High-Fat Diet Worsens Necrotizing Enterocolitis via Effects on Mitochondrial Function in Newborn Mice.

Koichi Tsuboi, Hee-Seong Jang, Pranav Prakash, Brian Kwon, Sanxia Wang, Menghan Wang, Thomas Prindle, William B Fulton, Chhinder P Sodhi, David J Hackam

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In one paragraph

Article in Cellular and molecular gastroenterology and hepatology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

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No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Koichi TsuboiDivision of Pediatric Surgery, Department of Surgery, Johns Hopkins University School of Medicine, and the Johns Hopkins Children's Center, Baltimore, Maryland.
Hee-Seong JangDivision of Pediatric Surgery, Department of Surgery, Johns Hopkins University School of Medicine, and the Johns Hopkins Children's Center, Baltimore, Maryland.
Pranav PrakashDivision of Pediatric Surgery, Department of Surgery, Johns Hopkins University School of Medicine, and the Johns Hopkins Children's Center, Baltimore, Maryland.
Brian KwonDivision of Pediatric Surgery, Department of Surgery, Johns Hopkins University School of Medicine, and the Johns Hopkins Children's Center, Baltimore, Maryland.
Sanxia WangDivision of Pediatric Surgery, Department of Surgery, Johns Hopkins University School of Medicine, and the Johns Hopkins Children's Center, Baltimore, Maryland.
Menghan WangDivision of Pediatric Surgery, Department of Surgery, Johns Hopkins University School of Medicine, and the Johns Hopkins Children's Center, Baltimore, Maryland.
Thomas PrindleDivision of Pediatric Surgery, Department of Surgery, Johns Hopkins University School of Medicine, and the Johns Hopkins Children's Center, Baltimore, Maryland.
William B FultonDivision of Pediatric Surgery, Department of Surgery, Johns Hopkins University School of Medicine, and the Johns Hopkins Children's Center, Baltimore, Maryland.
Chhinder P SodhiDivision of Pediatric Surgery, Department of Surgery, Johns Hopkins University School of Medicine, and the Johns Hopkins Children's Center, Baltimore, Maryland. Electronic address: csodhi@jhmi.edu.
David J HackamDivision of Pediatric Surgery, Department of Surgery, Johns Hopkins University School of Medicine, and the Johns Hopkins Children's Center, Baltimore, Maryland. Electronic address: Dhackam1@jhmi.edu.

Funding

Prenatal and Postnatal Mitochondrial Dysfunction in the Pathogenesis of Necrotizing EnterocolitisR01DK141072 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI HACKAM, DAVID J · 2025 to 2025
$2.3M
NIDDK NIH HHS R01 DK141072
6 · The paper itself

Abstract

BACKGROUND &

aimsNecrotizing enterocolitis (NEC) is a life-threatening intestinal disease of preterm infants that causes epithelial injury and necrosis. Prematurity and postnatal formula administration are known contributors, but maternal nutrition may also influence offspring gut susceptibility. The biological relationship between maternal diet and neonatal intestinal vulnerability remains unclear.

methodsA maternal high-fat diet (HFD) mouse model with a neonatal NEC model were used to investigate how maternal HFD affects offspring outcomes. Bulk RNA sequencing of ileal tissue before NEC induction was used to assess baseline transcription profiles. Following NEC, survival rate was assessed with ileal histological injury, epithelial reactive oxygen species, and mitochondrial membrane depolarization, as well as ultrastructural characteristics on transmission electron microscopy.

resultsCompared with standard diet, offspring born from HFD mothers showed altered mitochondria-related gene expression, shorter villi, and reduced MUC2

conclusionsMaternal HFD disrupts mitochondrial architecture and transcriptional programming in the neonatal intestine, promoting a pre-injured state that increases NEC susceptibility, suggesting that maternal diet may be a modifiable target for early NEC prevention.

Indexed as

Diet, High-FatEnterocolitis, NecrotizingMitochondriaPrenatal Exposure Delayed EffectsAnimalsAnimals, NewbornDevelopmental Origins of Health and DiseaseDisease Models, AnimalFemaleIleumIntestinal MucosaMiceMice, Inbred C57BLPregnancyReactive Oxygen SpeciesReactive Oxygen SpeciesDietMaternal Diabetes MellitusPrematuritySepsisToll-Like Receptor

Identifiers

PMID41997361
PMCPMC13276422

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.