ArticleCell reports methods2026
HAK-actin, a U-ExM-compatible probe to image the actin cytoskeleton.
Article in Cell reports methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Molecular and cellular insights into the parasitic infection of bloom-forming marine diatoms by Pirsonia diadema.Nature microbiology · 2026Article
- HAK-actin brings F-actin into focus in expansion microscopy.Nature reviews. Molecular cell biology · 2026Article
- Molecular evidence for early deuterostome origins of ovarian cell types and neuroendocrine control of reproduction.Nature communications · 2026Article
- IntAct-U-ExM enables super-resolution imaging of isoform-specific actin networks across species.PLoS biology · 2026Article
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Authors and funding
10 authors.
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Abstract
Expansion microscopy (ExM) enables super-resolution imaging by physically enlarging biological samples. While ExM has been successfully applied to study the intracellular microtubule cytoskeleton, reliable probes for visualizing actin fibers remain limited. Here, we present HAK-actin, an engineered actin probe compatible with ultrastructure expansion microscopy (U-ExM). We show that HAK-actin delivers robust and uniform actin staining across diverse systems, including human cells, microbial eukaryotes, and mouse retinal tissue. This tool provides a simple, versatile, and reproducible solution for actin cytoskeleton visualization, addressing a critical need in cell biology.
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