Protocol update to: Genome-wide interrogation of protein-DNA interactions in mammalian cells using ChIPmentation.

Xuemei Li, Wei Xu, Ying Ye, Andrew D Sharrocks, Wensheng Zhang, Xi Chen

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In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Xuemei LiSchool of Basic Medical Sciences, Shandong Medical and Pharmaceutical University, No. 318 Gangcheng East Street, Laishan District, Yantai, Shandong 264003, China.

Wei XuGMU-GIBH Joint School of Life Sciences, Guangdong Provincial Key Laboratory of Protein Modification and Disease, The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and Diseases, Guangzhou Medical University, Zibo, Guangdong 511436, China.

Ying YeDepartment of Clinical Pathobiology and Immunological Testing, School of Medical Laboratory, Qilu Medical University, Zibo 255300, China.

Andrew D SharrocksFaculty of Biology, Medicine and Health, University of Manchester, Oxford Rd, Manchester M13 9PL, UK.

Wensheng ZhangSchool of Basic Medical Sciences, Shandong Medical and Pharmaceutical University, No. 318 Gangcheng East Street, Laishan District, Yantai, Shandong 264003, China; School of Life Sciences and Medicine, Shandong University of Technology, Zibo, Shandong 255000, China. Electronic address: zhangwensheng@suda.edu.cn.

Xi ChenShenzhen Key Laboratory of Gene Regulation and Systems Biology, Department of Systems Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China. Electronic address: chenx9@sustech.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mapping the genomic locations of chromatin-associated proteins, such as transcription factors and histone modifications, is key to understanding the mechanisms of transcriptional regulation. ChIPmentation offers a simple and robust way of investigating the genomic binding sites of a protein using relatively low-input material. Here, we present a detailed protocol for the key steps that lead to a successful ChIPmentation experiment, as well as a quick analysis pipeline to examine the data. For complete details on the use and execution of this protocol, please refer to Schmidl et al.

Indexed as

Chromatin ImmunoprecipitationDNADNA-Binding ProteinsAnimalsBinding SitesChromatinGenomeHumansProtein BindingTranscription FactorsChromatinDNADNA-Binding ProteinsTranscription FactorsChIPChromatin immunoprecipitationGenomicsMolecular Biology

Identifiers

PMID41996248

DOI10.1016/j.xpro.2026.104495

PMCPMC13098593

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.