Evidence map›Paper›PMID 41995904›Full record

ArticleArchives of virology2026

A novel TaqMan-based RT-qPCR assay for the detection of PEDV and discrimination of the G2c subtype.

Yicong Fan, Xiang Li, Jiawen Mo, Liang Weng, Shiyu Liu, Xu Song, Rongli Guo, Wei Wang, Mi Hu, Shanshan Yang and 5 more

Abstract read
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In one paragraph

Article in Archives of virology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Yicong FanSchool of Veterinary Medicine, Hebei Agricultural University, Baoding, China.
Xiang LiInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, Nanjing, China.
Jiawen MoInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, Nanjing, China.
Liang WengInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, Nanjing, China.
Shiyu LiuInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, Nanjing, China.
Xu SongInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, Nanjing, China.
Rongli GuoInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, Nanjing, China.
Wei WangInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, Nanjing, China.
Mi HuInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, Nanjing, China.
Shanshan YangInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, Nanjing, China.
Yongxiang ZhaoInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, Nanjing, China.
Baochao FanInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, Nanjing, China.
Bin LiSchool of Veterinary Medicine, Hebei Agricultural University, Baoding, China. libinana@126.com.
Min SunInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, Nanjing, China. sunmin9084@aliyun.com.
Junming ZhouInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, Nanjing, China. zhoujm0724@163.com.ORCID http://orcid.org/0009-0006-1897-3718

Funding

Jiangsu Agricultural Science and Technology Innovation Fund CX(24)3071Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation Open Project JSYSZJ2024001National Key Research and Development Program of China 2025YFD1800903National Natural Science Foundation of China 32373030Natural Science Foundation of Jiangsu Province BK20230077, BE2023317Regional Innovation and Development Joint Fund of National Natural Science Foundation of China U23A20236,U25A20716
6 · The paper itself

Abstract

Porcine epidemic diarrhea virus (PEDV) G2c subtype has emerged as an increasingly prevalent variant causing widespread epidemic, high mortality and severe economic losses in the Chinese swine industry. The lack of specific and efficient detection methods hinders its surveillance, early detection and control. In this study, a TaqMan-MGB probe-based duplex quantitative real-time reverse transcription PCR (RT-qPCR) assay was developed for simultaneous detection of pan-PEDV and differentiation of the G2c subtype. Universal primers/probe targeting the conserved N gene of PEDV and G2c-specific primers/probe targeting the unique mutation sites in the S gene were designed. The assay was validated for performance, including specificity, sensitivity, and repeatability, and further evaluated using artificial challenge models and clinical samples. The standard curves exhibited excellent linearity (R

Indexed as

Coronavirus InfectionsPorcine epidemic diarrhea virusReal-Time Polymerase Chain ReactionSwine DiseasesAnimalsChinaDNA PrimersFecesLimit of DetectionReproducibility of ResultsSensitivity and SpecificitySwineDNA Primers

Identifiers

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.