ArticleBioMed research international2026
Molecular Characterization of Staphylococcus aureus and Coagulase-Negative Staphylococci Isolates at a Rural Hospital in Southern Malawi.
Article in BioMed research international, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Molecular Characterization of Staphylococcus aureus and Coagulase-Negative Staphylococci Isolates at a Rural Hospital in Southern Malawi.BioMed research international · 2026Article
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4 authors.
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Abstract
objectivesThis study is aimed at identifying antimicrobial resistance (AMR) and virulence-encoded genes in Staphylococcus aureus and coagulase-negative staphylococci (CoNS) isolates from a rural hospital in Southern Malawi. S. aureus isolates were further characterized by typing of staphylococcal protein A (spa).
methodsWe conducted a cross-sectional study of 36 Staphylococcus isolates from clinical samples collected at Malamulo Hospital in Makwasa, Malawi. Antimicrobial susceptibility testing was performed using the disc diffusion method. Polymerase chain reaction (PCR) was used to detect AMR genes (mecA, ermA, ermB, ermC, tetK, tetL, tetM, and tetO) and virulence genes (PVL: lukS-PV, lukF-PV; splA, splB, splC, splD, splE, and splF). PCR also confirmed S. aureus isolates through the detection of nuc, spa, or coa genes. Spa typing of S. aureus isolates was performed by Sanger sequencing.
resultsThe 36 isolates were identified as 14 S. aureus and 22 CoNS species. The highest resistance among all isolates was against sulfamethoxazole/trimethoprim (n = 18, χ
conclusionThis study reveals the presence of both multidrug-resistant CoNS and S. aureus strains with diverse spa types in rural Southern Malawi and highlights a role for molecular testing in surveillance and diagnostic testing.
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