Evidence map›Paper›PMID 41994107›Full record

ArticleResearch square2026

The tumour suppressor RBM5 activates the helicase DHX15 to regulate splicing.

Shiheng Liu, Tiantian Su, Jeffrey Huang, Chia-Ho Lin, Douglas L Black, Andrey Damianov, Z Hong Zhou

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Shiheng LiuDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Tiantian SuDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.ORCID https://orcid.org/0000-0002-9412-5503
Jeffrey HuangDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Chia-Ho LinDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Douglas L BlackDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.ORCID https://orcid.org/0000-0002-2705-8187
Andrey DamianovDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.ORCID 0009-0001-9845-913X
Z Hong ZhouDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.ORCID 0000-0002-8373-4717

Funding

UCLA Clinical Translational Science InstituteUL1TR001881 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ARLEEN F. BROWN, ARASH NAEIM · 2016 to 2026
$118.1M
Mechanisms of Post-transcriptional Gene Regulation by RNA Binding ProteinsR35GM136426 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Douglas L Black · 2020 to 2026
$5.4M
High-Resolution CryoEM Reconstruction of Large ComplexesR01GM071940 · NIGMS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI ZHOU, Z HONG · 2006 to 2025
$5.0M
Identification of novel splicing regulatory complexesR01GM127473 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DAMIANOV, ANDREY · 2019 to 2023
$1.6M
HIGH-END CRYOEM INSTRUMENT FOR UCLA ELECTRON IMAGING CTR FOR NANOMACHINES: AIDSS10RR023057 · NCRR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHOU, Z HONG · 2006 to 2006
$1.6M
Direct Detection Device for atomic resolution cryoEM of macromolecular complexesS10OD018111 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHOU, Z HONG · 2014 to 2014
$598k
NCATS NIH HHS UL1 TR001881NCRR NIH HHS S10 RR023057NIGMS NIH HHS R01 GM071940NIGMS NIH HHS R01 GM127473NIGMS NIH HHS R35 GM136426NIH HHS S10 OD018111
6 · The paper itself

Abstract

Pre-mRNA splicing determines the expressed proteome and is frequently dysregulated in cancer. The tumour-suppressor RBM5 controls an exon network regulating apoptosis, yet its molecular mechanism is elusive. Using in vivo spliceosome capture and cryogenic electron microscopy, we determined structures of precatalytic spliceosomes arrested by RBM5 immediately after U2 snRNP branchpoint recognition. Despite intron diversity, the U2-pre-mRNA duplex, branchpoint adenine, and downstream polypyrimidine tract are well-resolved. RBM5 binds the outer SF3B1 HEAT surface and performs dual functions: First, its helix-loop-helix motif and upstream zinc-finger domain sterically block tri-snRNP and Prp8 docking and prevent progression to pre-B and B

Identifiers

PMID41994107
PMCPMC13082122

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.