Evidence map›Paper›PMID 41993518›Full record

ArticlebioRxiv : the preprint server for biology2026

Development and validation of two economical and flexible immunoassays for detecting antibodies against LCMV in mouse serum.

Rebekah Honce, Jillian German, Ella K Botten, Chloe Schiff, Emily Van Beek, Allysen Henriksen, Kanayo Ikeh, Akshay Neeli, Philip Eisenhauer, Inessa Manuelyan and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Rebekah HonceDepartment of Medicine, Division of Pulmonary and Critical Care Medicine, Larner College of Medicine, University of Vermont, Burlington, VT 05405.ORCID 0000-0001-5633-9116
Jillian GermanDepartment of Medicine, Division of Pulmonary and Critical Care Medicine, Larner College of Medicine, University of Vermont, Burlington, VT 05405.
Ella K BottenDepartment of Medicine, Division of Pulmonary and Critical Care Medicine, Larner College of Medicine, University of Vermont, Burlington, VT 05405.
Chloe SchiffDepartment of Medicine, Division of Pulmonary and Critical Care Medicine, Larner College of Medicine, University of Vermont, Burlington, VT 05405.
Emily Van BeekDepartment of Medicine, Division of Pulmonary and Critical Care Medicine, Larner College of Medicine, University of Vermont, Burlington, VT 05405.ORCID 0009-0005-8878-6435
Allysen HenriksenDepartment of Medicine, Division of Pulmonary and Critical Care Medicine, Larner College of Medicine, University of Vermont, Burlington, VT 05405.
Kanayo IkehDepartment of Medicine, Division of Pulmonary and Critical Care Medicine, Larner College of Medicine, University of Vermont, Burlington, VT 05405.
Akshay NeeliCellular, Molecular, and Biomedical Sciences Graduate Program, University of Vermont, Burlington, VT 05405.
Philip EisenhauerDepartment of Medicine, Division of Pulmonary and Critical Care Medicine, Larner College of Medicine, University of Vermont, Burlington, VT 05405.
Inessa ManuelyanDepartment of Medicine, Division of Pulmonary and Critical Care Medicine, Larner College of Medicine, University of Vermont, Burlington, VT 05405.
Jason W BottenDepartment of Medicine, Division of Pulmonary and Critical Care Medicine, Larner College of Medicine, University of Vermont, Burlington, VT 05405.ORCID 0000-0001-6839-0306

Funding

Multidisciplinary Training in Lung BiologyT32HL076122 · NHLBI · UNIVERSITY OF VERMONT &ST AGRIC COLLEGE · PI IRVIN, CHARLES G, POYNTER, MATTHEW E · 2004 to 2023
$6.1M
The role of mammarenavirus defective interfering particles in protecting host fitness and the host-driven post-translational modifications that regulate their formation and functionR01AI171408 · NIAID · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI Jason W. Botten · 2022 to 2026
$2.9M
NHLBI NIH HHS T32 HL076122NIAID NIH HHS R01 AI171408
6 · The paper itself

Abstract

Measurement of antibody responses to viral infection is essential for surveillance, diagnostics, epidemiological research, and natural history of infection studies. However, current methods to detect virus-specific antibodies are often resource-intensive and impractical for deployment in outbreak settings or in field-based studies. This manuscript presents two economical, high-throughput immunoassays-the cytoblot immunoassay (CBA) and strip immunoblot assay (SIA)-for detecting and quantifying anti-lymphocytic choriomeningitis mammarenavirus (LCMV) antibodies in mouse serum. To validate, we tested serum from acutely or persistently experimentally infected mice. Both assays detected LCMV-specific IgG and IgM antibodies with high sensitivity and specificity across multiple timepoints. By facilitating the study of immune responses in rodent reservoirs, these tools can enhance our understanding of zoonotic viral transmission, provide scalable platforms for outbreak preparedness, and serve as adaptable models for the development of rapid serological assays for other viral pathogens.

Identifiers

PMID41993518
PMCPMC13081961

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.