Evidence map›Paper›PMID 41993375›Full record

ArticlebioRxiv : the preprint server for biology2026

Chemotherapy-Induced Oral Mucosal Injury Is Defined by p53 Activation, Cell Cycle Arrest and Diverse Epithelial Progenitor Dynamics.

Pedro H F Silva, Lu Li, Madison Muriel, Tara Brennan, Md Hasanuzzaman, Mariadela Demoura, Joleene Stampfer, Michele Sveinsson, Christos Fountzilas, Jill E Schabert and 5 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Pedro H F SilvaDepartment of Oral Biology, University at Buffalo, The State University of New York, Buffalo, New York, USA.
Lu LiDepartment of Oral Biology, University at Buffalo, The State University of New York, Buffalo, New York, USA.
Madison MurielDepartment of Oral Biology, University at Buffalo, The State University of New York, Buffalo, New York, USA.
Tara BrennanDepartment of Oral Biology, University at Buffalo, The State University of New York, Buffalo, New York, USA.
Md HasanuzzamanDepartment of Oral Biology, University at Buffalo, The State University of New York, Buffalo, New York, USA.
Mariadela DemouraDepartment of Oral Biology, University at Buffalo, The State University of New York, Buffalo, New York, USA.
Joleene StampferDepartment of Cancer Prevention & Control, Roswell Park Comprehensive Cancer Center, Buffalo, New York, USA.
Michele SveinssonDepartment of Oral Biology, University at Buffalo, The State University of New York, Buffalo, New York, USA.
Christos FountzilasDepartment of Medicine, Roswell Park Comprehensive Cancer Center, Buffalo, New York, USA.
Jill E SchabertDepartment of Dentistry and Maxillofacial Prosthetics, Roswell Park Comprehensive Cancer Center, Buffalo, New York, USA.
Anurag K SinghDepartment of Radiation Medicine, Roswell Park Comprehensive Cancer Center, Buffalo, New York, USA.
Jonathan E BardDepartment of Biochemistry, University at Buffalo, The State University of New York, Buffalo New York, USA.
Nicolas F SchlechtDepartment of Cancer Prevention & Control, Roswell Park Comprehensive Cancer Center, Buffalo, New York, USA.
Laertis IkonomouDepartment of Oral Biology, University at Buffalo, The State University of New York, Buffalo, New York, USA.
Patricia I DiazDepartment of Oral Biology, University at Buffalo, The State University of New York, Buffalo, New York, USA.

Funding

Host and microbial risk factors of oral thrush in cancer patients receiving chemotherapyR01DE032131 · NIDCR · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI Patricia Diaz, NICOLAS SCHLECHT · 2022 to 2026
$3.7M
Mechanisms of Cell Death and Inflammation in Chemotherapy-Induced Oral MucositisR56DE028545 · NIDCR · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI DIAZ, PATRICIA · 2020 to 2020
$657k
Using Novel Machine Learning Approaches to Understand Subgingival Microbiome Heterogeneity in Relation to Health and DiseaseK99DE034829 · NIDCR · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI Lu Li · 2025 to 2026
$243k
NIDCR NIH HHS K99 DE034829NIDCR NIH HHS R01 DE032131NIDCR NIH HHS R56 DE028545
6 · The paper itself

Abstract

Chemotherapy-induced oral mucositis is a common and debilitating complication, yet the mechanisms underlying oral mucosa injury and repair remain poorly defined. Using a mouse model of 5-fluorouracil (5-FU)-induced mucositis, we define gene networks and oral mucosal cellular landscape dynamics in response to chemotoxic stress. We show that 5-FU-induced epithelial atrophy is driven primarily by cell cycle arrest rather than apoptosis, despite concurrent activation of p53-dependent transcriptional programs linked to both cell fates within individual cells. Relative to intestine, the recovering oral mucosa exhibits a more effective cell cycle checkpoint response and uniquely undergoes metabolic reprogramming toward lipid oxidation. Single-cell RNA sequencing revealed putative epithelial progenitor populations with distinct responses to chemotherapy, including chemoresistant cells with a p53- and AP-1 complex gene signature, reminiscent of lung transitional cell states. These findings define diverse progenitor dynamics and p53-driven responses as key determinants of oral mucosal injury and repair following chemotherapy.

Identifiers

PMID41993375
PMCPMC13081878

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.