Evidence map›Paper›PMID 41993343›Full record

ArticlebioRxiv : the preprint server for biology2026

Suppression of upstream ORF translation is not a widespread mechanism of translational stimulation by yeast helicase Ded1.

Rakesh Kumar, Gemma May, Neelam Dabas Sen, C Joel McManus, Alan G Hinnebusch

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Rakesh KumarDivision of Molecular and Cellular Biology, Eunice K. Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
Gemma MayDepartment of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, 15213, USA.
Neelam Dabas SenDivision of Molecular and Cellular Biology, Eunice K. Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
C Joel McManusDepartment of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, 15213, USA.
Alan G HinnebuschDivision of Molecular and Cellular Biology, Eunice K. Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.

Funding

Regulation of mRNA translation by cis-acting sequences and trans-acting factorsR35GM145317 · NIGMS · CARNEGIE-MELLON UNIVERSITY · PI Charles Joel McManus · 2022 to 2026
$1.9M
NIGMS NIH HHS R35 GM145317
6 · The paper itself

Abstract

Ded1 is an essential DEAD-box helicase in yeast that broadly stimulates translation initiation and is critical for mRNAs with structured 5'UTRs. We have evaluated the proposal that Ded1 stimulates translation primarily by preventing initiation at upstream ORFs (uORFs) associated with stable secondary structures. By Ribo-Seq analysis under experimental conditions designed to suppress artifactual 5'UTR translation, we found that reduced translation of the main open-reading-frames (mORFs) in native mRNAs is generally not accompanied by increased 5'UTR translation in

Indexed as

Ded1/DDX3XFACS-uORFribosomeRNA helicaseupstream open-reading frames

Identifiers

PMID41993343
PMCPMC13082114

What OpenQuestion holds

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LicenceCC0
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.