Evidence map›Paper›PMID 41993337›Full record

ArticlebioRxiv : the preprint server for biology2026

Adapting Upright Light Sheet Fluorescence Microscopy for Imaging at Air-Liquid Interface.

Chad M Hobson, Kenji Izumi, Jesse S Aaron, Navaneetha Krishnan Bharathan, M Fernanda Ceriani, William Giang, Juan I Ispizua, Andrew P Kowalczyk, Rachel M Lee, E Angelo Morales and 9 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

19 authors.

Chad M HobsonAdvanced Imaging Center, Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Kenji IzumiTOKAI HIT CO., LTD., Fujinomiya-shi, Shizuoka-ken, Japan.
Jesse S AaronAdvanced Imaging Center, Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Navaneetha Krishnan BharathanThe Pennsylvania State University College of Medicine, Hershey, PA, USA.
M Fernanda CerianiLaboratorio de Genética del Comportamiento. Fundación Instituto Leloir, IIB-BA/CONICET, Buenos Aires, Argentina.
William GiangThe Pennsylvania State University College of Medicine, Hershey, PA, USA.
Juan I IspizuaLaboratorio de Genética del Comportamiento. Fundación Instituto Leloir, IIB-BA/CONICET, Buenos Aires, Argentina.
Andrew P KowalczykThe Pennsylvania State University College of Medicine, Hershey, PA, USA.
Rachel M LeeAdvanced Imaging Center, Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
E Angelo MoralesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Owen F PulsIntegrative Imaging, Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Ellen QuarlesIntegrative Imaging, Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Micaela Rodriguez-CaronLaboratorio de Genética del Comportamiento. Fundación Instituto Leloir, IIB-BA/CONICET, Buenos Aires, Argentina.
Sara N StahleyThe Pennsylvania State University College of Medicine, Hershey, PA, USA.
Francisco TassaraLaboratorio de Genética del Comportamiento. Fundación Instituto Leloir, IIB-BA/CONICET, Buenos Aires, Argentina.
Shaohe WangJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Shunze YaoJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Takanori TsuchiyaTOKAI HIT CO., LTD., Fujinomiya-shi, Shizuoka-ken, Japan.
Teng-Leong ChewAdvanced Imaging Center, Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.

Funding

Role of p0071 in Cutaneous Intercellular JunctionsR01AR048266 · NIAMS · PENNSYLVANIA STATE UNIV HERSHEY MED CTR · PI ANDREW P. KOWALCZYK · 2002 to 2026
$8.2M
Circadian Structural Plasticity in Central PacemakersR01NS108934 · NINDS · UNIVERSITY OF WASHINGTON · PI CERIANI, MARIA FERNANDA, DE LA IGLESIA, HORACIO O · 2020 to 2024
$2.9M
Keratinocyte adhesion and signaling in the skin blistering disease pemphigus vulgarisR01AR081883 · NIAMS · PENNSYLVANIA STATE UNIV HERSHEY MED CTR · PI ANDREW P. KOWALCZYK · 2023 to 2026
$2.3M
Cadherin regulation of planar polarityR35GM150921 · NIGMS · PENNSYLVANIA STATE UNIV HERSHEY MED CTR · PI Sara N Stahley · 2023 to 2026
$1.6M
NIAMS NIH HHS R01 AR048266NIAMS NIH HHS R01 AR081883NIGMS NIH HHS R35 GM150921NINDS NIH HHS R01 NS108934
6 · The paper itself

Abstract

Light sheet fluorescence microscopy (LSFM) is increasingly appreciated as the gold standard for gentle, volumetric imaging with fast acquisition speeds and/or long imaging durations. However, the often-constrained sample space of these microscopes has precluded a specific class of biological specimens from being studied with these tools: those requiring an air-liquid interface (ALI). Here, we present a device for robust imaging at ALI on an upright light sheet microscope with dipping objectives. We demonstrate the system using three relevant use-cases:

Identifiers

PMID41993337
PMCPMC13082110

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.