Evidence map›Paper›PMID 41993335›Full record

ArticlebioRxiv : the preprint server for biology2026

RNA polymerase inhibitors reveal active-site motions essential for the nucleotide-addition cycle.

Yukti Dhingra, Robert Landick, Elizabeth A Campbell, Seth A Darst

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Yukti DhingraLaboratory of Molecular Biophysics, The Rockefeller University, New York, USA.ORCID 0000-0001-9060-8201
Robert LandickDepartment of Biochemistry and Department of Bacteriology, University of Wisconsin-Madison, Wisconsin, USA.ORCID 0000-0002-5042-0383
Elizabeth A CampbellLaboratory of Molecular Pathogenesis, The Rockefeller University, New York, USA.ORCID 0000-0002-1332-128X
Seth A DarstLaboratory of Molecular Biophysics, The Rockefeller University, New York, USA.ORCID 0000-0002-8241-3153

Funding

Structure, function, and regulation of the bacterial transcription cycleR35GM118130 · NIGMS · ROCKEFELLER UNIVERSITY · PI DARST, SETH A. · 2016 to 2025
$8.9M
EXTRACELLULAR MATRIX PROTEIN ENHANCEMENT OF PHAGOCYTOSISR01GM038330 · NIGMS · WASHINGTON UNIVERSITY · PI VONZASTROW, MARK E · 1987 to 2007
$2.7M
Structural and functional studies of the mycobacterial transcription cycleR35GM151879 · NIGMS · ROCKEFELLER UNIVERSITY · PI ELIZABETH A CAMPBELL · 2024 to 2026
$1.4M
NIGMS NIH HHS R01 GM038330NIGMS NIH HHS R35 GM118130NIGMS NIH HHS R35 GM151879
6 · The paper itself

Abstract

The nucleotide-addition cycle (NAC) of multi-subunit DNA-dependent RNA polymerases (RNAPs) involves coordinated conformational changes in conserved active-site structural elements, including the trigger loop (TL). The TL is open (unfolded) in most RNAP structures but can close (fold) in substrate-bound (post- or pre-translocated) states of the RNAP, promoting catalysis. TL closure has been associated with closure of another conserved structural element, the Rim-Helices/F-loop (RH-FL), but the role of the RH-FL in the NAC is unclear. Antibiotic leads CBR9379 and AAP-SO

Indexed as

Antibiotic mechanismNucleotide additionRNA polymeraseTranscriptionTrigger loop

Identifiers

PMID41993335
PMCPMC13081874

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.