Evidence map›Paper›PMID 41993286›Full record

ArticlebioRxiv : the preprint server for biology2026

A versatile, positive-going voltage indicator that enables accessible two-photon recordings in vivo.

Alex James McDonald, Michelle Ann Land, Shuyuan Yang, Noura Hakam, Vincent Villette, Jun Zhu, Mario Galdamez, Mario Fernandez de La Puebla, Xiaoyu Lu, Gregory Foran and 25 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

35 authors.

Alex James McDonaldDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Michelle Ann LandDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Shuyuan YangDepartment of Chemical and Biomolecular Engineering, Rice University, Houston, TX, 77005, USA.
Noura HakamDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Vincent VilletteInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, Université PSL, Paris F-75005, France.
Jun ZhuDepartment of Neuroscience, University of California, Berkeley, CA, 94720, USA.
Mario GaldamezDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Mario Fernandez de La PueblaKavli Institute for Systems Neuroscience, Trondheim, Norway.
Xiaoyu LuDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Gregory ForanDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Tanmayee Torne-SrivastavaDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Beatriz CampilloDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Haixin LiuDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Xiaoyu DongDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Shujuan LaiDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Matthew ShoreyDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Hala AbdallahDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Raegan BanksDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Anastasia MamontovaDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Yu-Yau York ShanDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Ryan KroegerDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Robert G LawDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Ming HuDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Daniela Gaspar SantosInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, Université PSL, Paris F-75005, France.
Jonathan BradleyInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, Université PSL, Paris F-75005, France.
Alberto LombardiniInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, Université PSL, Paris F-75005, France.
Benjamin MathieuInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, Université PSL, Paris F-75005, France.
Annick AyonInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, Université PSL, Paris F-75005, France.
Ryan Gregory NatanDepartment of Neuroscience, University of California, Berkeley, CA, 94720, USA.
Hongyi YuanDepartment of Physics, University of California, Berkeley, CA, 94720, USA.
Jacob ReimerDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Laurent BourdieuInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, Université PSL, Paris F-75005, France.
Na JiDepartment of Neuroscience, University of California, Berkeley, CA, 94720, USA.
Weijian ZongKavli Institute for Systems Neuroscience, Trondheim, Norway.
François St-PierreDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.ORCID 0000-0001-8618-4135

Funding

Molecular Engineering of Natural Light-Gated Chloride Channels for Optogenetic InhibitionU01NS118288 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI SPUDICH, JOHN LEE, ST-PIERRE, FRANCOIS · 2020 to 2023
$5.2M
ENABLING SUBMILLISECOND-TIMESCALE TWO-PHOTON RECORDING OF VOLTAGE DYNAMICS IN THREE DIMENSIONS IN VIVOU01NS133971 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI Jacob Reimer, Francois St-Pierre · 2023 to 2026
$4.0M
Designing and deploying an expanded color palette of voltage indicators engineered for multiphoton microscopyU01NS113294 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI ST-PIERRE, FRANCOIS, TOLIAS, ANDREAS · 2019 to 2021
$3.7M
Engineering photostable fluorescent proteins and biosensors using transcriptomic mining and massive-throughput single-cell screeningR01EB032854 · NIBIB · BAYLOR COLLEGE OF MEDICINE · PI ST-PIERRE, FRANCOIS · 2022 to 2025
$2.4M
The Spatial and Temporal Scale of Neuromodulation in Mouse Sensory CortexRF1NS128901 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI REIMER, JACOB · 2022 to 2022
$2.3M
Engineering designer probes for imaging membrane potential: novel parts, architectures, and platformsR01EB027145 · NIBIB · BAYLOR COLLEGE OF MEDICINE · PI ST-PIERRE, FRANCOIS · 2019 to 2022
$2.2M
Optimizing FACED for robust large-scale activity recordingU01NS137449 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI NA Ji · 2024 to 2026
$2.1M
Adaptive optical microscopy for high-accuracy recording of neural activity in vivoU01NS118300 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI JI, NA · 2021 to 2023
$1.7M
Red-shifted voltage indicators for two-photon all-optical physiology and multi-spectral imagingR01NS136027 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI Jacob Reimer, Francois St-Pierre · 2024 to 2026
$1.7M
The Spatial and Temporal Scale of Neuromodulation in Mouse Sensory CortexR01NS128901 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI Jacob Reimer · 2025 to 2026
$1.4M
IN VIVO IMAGING OF STATE-DEPENDENT ASTROCYTE VOLTAGE DYNAMICS AND NEURAL ACTIVITY IN MOUSE VISUAL CORTEXR01NS146023 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI Jacob Reimer, Francois St-Pierre · 2025 to 2026
$1.3M
Advanced Voltage Indicators Tailored for Two-Photon Resonant Scanning ApplicationsR01NS146078 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI Jacob Reimer, Francois St-Pierre · 2025 to 2026
$1.3M
NIBIB NIH HHS R01 EB027145NIBIB NIH HHS R01 EB032854NINDS NIH HHS R01 NS128901NINDS NIH HHS R01 NS136027NINDS NIH HHS R01 NS146023NINDS NIH HHS R01 NS146078NINDS NIH HHS R34 NS132045NINDS NIH HHS RF1 NS128901NINDS NIH HHS U01 NS113294NINDS NIH HHS U01 NS118288NINDS NIH HHS U01 NS118300NINDS NIH HHS U01 NS133971NINDS NIH HHS U01 NS137449
6 · The paper itself

Abstract

Genetically encoded voltage indicators (GEVIs) enable cell‑type-specific optical readout of membrane potential, but two‑photon (2P) spike detection has been hampered by low signal‑to‑noise and ultrafast off‑kinetics, restricting use to specialized microscopes. We introduce FORCE1s, a green, positive‑going GEVI engineered to make robust 2P voltage imaging broadly accessible. FORCE1s brightens from a dark baseline during depolarization, reports spikes with ~100%

Identifiers

PMID41993286
PMCPMC13082015

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.