Evidence map›Paper›PMID 41992084›Full record

ArticleBMC genomics2026

Multi-omics analysis reveals seasonal variation in ovarian lipid metabolism associated with vitamin D

Wenjing Lu, Yuyue Dong, Zhaomei Dong, Yuning Xie, Haolin Zhang, Yingying Han, Dong Zhang, Yuning Liu, Qiang Weng

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In one paragraph

Article in BMC genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Wenjing LuCollege of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, China.
Yuyue DongCollege of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, China.
Zhaomei DongCollege of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, China.
Yuning XieCollege of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, China.
Haolin ZhangCollege of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, China.
Yingying HanCollege of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, China.
Dong ZhangSchool of Ecology and Nature Conservation, Beijing Forestry University, Beijing, 100083, China.
Yuning LiuCollege of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, China. yuningliu9@bjfu.edu.cn.
Qiang WengCollege of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, China. qiangweng@bjfu.edu.cn.

Funding

5·5 Engineering Research & Innovation Team Project of Beijing Forestry University No. BLRC 2023C02Beijing Municipal Natural Science Foundation 7232240Fundamental Research Funds for the Central Universities NO. BLX202219National Undergraduate Training Programs for Innovation and Entrepreneurship 202410022032
6 · The paper itself

Abstract

backgroundSeasonal reproduction is critical for animals adapting to environmental changes, yet the mechanisms driving these shifts remain incompletely understood. Gut microbiota are increasingly recognized as important modulators of reproductive health, but their roles in seasonal breeding species remain unclear.

resultsIn the muskrat (Ondatra zibethicus), a well-established seasonal breeder, morphological and histological analyses revealed enhanced follicular development during the breeding season (BS). Subsequent integrated ovarian transcriptomics and metabolomics identified that lipid metabolic processes were significantly enriched during the BS. This was validated by the increased expression of key lipid metabolic enzymes (e.g., DGAT1, CPT1A) and elevated triglyceride content in BS ovaries. Systemically, we found that serum vitamin D3 (VD3) and ovarian vitamin D receptor (VDR) expression were significantly elevated during BS. In contrast, analysis of upstream factors during the non-breeding season (NBS) showed a significant enrichment of gut Lactobacillus and a concurrent elevation of plasma lithocholic acid (LCA), a known VDR agonist. Correlation analyses confirmed that both Lactobacillus and LCA were inversely associated with serum VD3 levels. Critically, in vitro experiments demonstrated that VD3 treatment enhanced the expression of key lipid metabolism genes in muskrat granulosa cells.

conclusionsCollectively, our findings provide evidence for a gut microbiota-VD3-ovary axis that modulates seasonal ovarian lipid metabolism and function. This axis represents a new layer of host-microbiota interaction in reproductive physiology.

Indexed as

ArvicolinaeCholecalciferolLipid MetabolismOvarySeasonsAnimalsFemaleGene Expression ProfilingMetabolomicsMultiomicsReceptors, CalcitriolTranscriptomeCholecalciferolReceptors, CalcitriolFollicle developmentGut microbiotaLipid metabolismMuskratVD3

Identifiers

PMID41992084
PMCPMC13202781

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.