Evidence map›Paper›PMID 41992038›Full record

ArticleNature biotechnology2026

Diversity-generating retroelements for programmable targeted hypermutagenesis.

Paul Rochette, Elena Lopez-Rodriguez, David J Wen, Léo Régnier, Catherine Fan, Anna Maikova, William Rostain, Linhan Wang, Imran Nooraddin, Amandine Maire and 12 more

Abstract read
PubMed Publisher
In one paragraph

Article in Nature biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Paul Rochette *Institut Pasteur, Synthetic Biology Unit, Université Paris Cité, Paris, France.
Elena Lopez-Rodriguez *Institut Pasteur, Synthetic Biology Unit, Université Paris Cité, Paris, France.
David J WenGladstone Institute of Data Science and Biology, San Francisco, CA, USA.ORCID http://orcid.org/0009-0008-8727-4253
Léo RégnierLaboratory of Physics of the Ecole Normale Superieure, PSL Research, CNRS UMR 8023, Sorbonne Université, Paris, France.ORCID http://orcid.org/0000-0002-3792-1570
Catherine FanInstitut Pasteur, Synthetic Biology Unit, Université Paris Cité, Paris, France.
Anna MaikovaInstitut Pasteur, Synthetic Biology Unit, Université Paris Cité, Paris, France.ORCID http://orcid.org/0000-0002-0222-9939
William RostainInstitut Pasteur, Synthetic Biology Unit, Université Paris Cité, Paris, France.ORCID http://orcid.org/0000-0002-9960-4669
Linhan WangGladstone Institute of Data Science and Biology, San Francisco, CA, USA.
Imran NooraddinInstitut Pasteur, Synthetic Biology Unit, Université Paris Cité, Paris, France.
Amandine MaireInstitut Pasteur, Synthetic Biology Unit, Université Paris Cité, Paris, France.
Paul VittotInstitut Pasteur, Synthetic Biology Unit, Université Paris Cité, Paris, France.
Nathan BarrabesInstitut Pasteur, Synthetic Biology Unit, Université Paris Cité, Paris, France.
Karol Melissa Cerdas-MejíasInstitut Pasteur, Synthetic Biology Unit, Université Paris Cité, Paris, France.
Auguste BouvierInstitut Pasteur, Synthetic Biology Unit, Université Paris Cité, Paris, France.
Thea ChrysostomouInstitut Pasteur, Synthetic Biology Unit, Université Paris Cité, Paris, France.
Orso SubriniInstitut Pasteur, Signaling and Dynamic of Receptors Unit, CNRS UMR3571, Université Paris Cité, Paris, France.
Nicolas WolffInstitut Pasteur, Signaling and Dynamic of Receptors Unit, CNRS UMR3571, Université Paris Cité, Paris, France.
Rémi MonassonLaboratory of Physics of the Ecole Normale Superieure, PSL Research, CNRS UMR 8023, Sorbonne Université, Paris, France.ORCID http://orcid.org/0000-0002-4459-0204
Simona CoccoLaboratory of Physics of the Ecole Normale Superieure, PSL Research, CNRS UMR 8023, Sorbonne Université, Paris, France.ORCID http://orcid.org/0000-0002-1852-7789
Seth L ShipmanGladstone Institute of Data Science and Biology, San Francisco, CA, USA.ORCID http://orcid.org/0000-0003-3130-8043
Raphael Laurenceau *Institut Pasteur, Synthetic Biology Unit, Université Paris Cité, Paris, France. raphael.laurenceau@pasteur.fr.ORCID http://orcid.org/0000-0002-1326-9928
David BikardInstitut Pasteur, Synthetic Biology Unit, Université Paris Cité, Paris, France. david.bikard@pasteur.fr.ORCID http://orcid.org/0000-0002-5729-1211

Funding

Agence Nationale de la Recherche (French National Research Agency) ANR-10-LABX-62-IBEIDEC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council) 101044479Fondation pour la Recherche Médicale (Foundation for Medical Research in France) ECO202206015569National Science Foundation (NSF) 2509382
6 · The paper itself

Abstract

Diversity-generating retroelements (DGRs) are natural systems that accelerate the evolution of diverse bacterial functions through targeted hypermutation. We establish a method using DGRs coupled to recombineering (DGRec), which enables the diversification of any sequence of interest in Escherichia coli. Detailed characterization of reverse transcriptase sequence biases demonstrates how it maximizes the exploration of the sequence space while avoiding nonsense mutations. By leveraging the high error rate of the DGR reverse transcriptase at adenines, DGRec can efficiently diversify user-defined sequence windows of 50-200 bp. Mutations can be focused at specific positions, with rates reaching up to 1.38 × 10

Identifiers

PMID41992038

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.