Evidence map›Paper›PMID 41991709›Full record

ArticleNeotropical entomology2026

Screening and Pathogenic Potential of Entomopathogenic Fungi Against Dactylopius opuntiae (Cockerell. 1929) (Hemiptera: Dactylopiidae).

Asmae Azzouzi, Hamza Anejjar, Siham Boutaib, Mohammed Baala, Youness Ighissane, Dina Akroute, Hajar El Hamss, Mustapha Ait Chitt, Abdelmalek Boutaleb Joutei, Rachid Lahlali and 1 more

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Article in Neotropical entomology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Asmae AzzouziPlant Protection Dept, Hassan II Institute of Agronomy and Veterinary Medicine (CEDoc-IAV), Rabat, Morocco.
Hamza AnejjarPlant Protection Dept, Hassan II Institute of Agronomy and Veterinary Medicine (CEDoc-IAV), Rabat, Morocco.
Siham BoutaibPlant Protection Dept, Agricultural Zoology Unit, National School of Agriculture of Meknes (ENAM), Meknes, Morocco.
Mohammed BaalaPlant Protection Dept, Phytopathology Unit, National School of Agriculture of Meknes (ENAM), Meknes, Morocco.
Youness IghissanePlant Protection Dept, Phytopathology Unit, National School of Agriculture of Meknes (ENAM), Meknes, Morocco.
Dina AkrouteRegional Center of Agricultural Research of Meknes, National Institute of Agricultural Research, Rabat, Morocco.
Hajar El HamssPlant Protection Dept, Phytopathology Unit, National School of Agriculture of Meknes (ENAM), Meknes, Morocco.
Mustapha Ait ChittLes Domaines Agricoles, Rabat, Morocco.
Abdelmalek Boutaleb JouteiPlant Protection Dept, Agricultural Zoology Unit, National School of Agriculture of Meknes (ENAM), Meknes, Morocco.
Rachid LahlaliPlant Protection Dept, Phytopathology Unit, National School of Agriculture of Meknes (ENAM), Meknes, Morocco. rlahlali@enameknes.ac.ma.ORCID http://orcid.org/0000-0002-1299-5733
Taoufiq BenzianePlant Protection Dept, Agricultural Zoology Unit, National School of Agriculture of Meknes (ENAM), Meknes, Morocco.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The cactus pear sector in Morocco remains seriously threatened by the cactus cochineal, Dactylopius opuntiae, the primary pest of Opuntia species worldwide. The objective of this study was to evaluate the entomopathogenic potential of fungi isolated from field-collected mycosed cochineal cadavers. Eleven fungal isolates were tested in the laboratory on adult female insects, leading to the selection of five isolates with particularly high efficacy: Fusarium equiseti (PV404064), Fusarium equiseti (PV404085), Aspergillus niger (PV706915), Aspergillus flavus (PV897714), and Alternaria destruens (PV403730). Estimation of LD50 values indicated that the five isolates exhibited similar levels of virulence. Tests conducted on infested cladodes confirmed the laboratory findings: 15 days after application, infestation rates were significantly reduced compared to the control. The study of mechanisms of action revealed diverse enzymatic activities: all tested fungi exhibited marked lipolytic, proteolytic, and phospholipase activities, whereas chitinolytic activity was detected only in the two F. equiseti isolates. Metabolic flexibility was also assessed: all isolates demonstrated 100% growth in minimal media supplemented with xylose and glucose compared to PDA medium. Finally, analysis of the secondary metabolism revealed the presence of several genes associated with the entomopathogenicity of the fungi against their hosts in the genomes of the five tested fungal isolates.

Indexed as

FungiHemipteraPest Control, BiologicalAnimalsFemaleCactus pearDactylopius opuntiaeEntomopathogenic fungiMorocco

Identifiers

PMID41991709

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.