ArticleAnnals of botany2026
A standardized protocol and basic ecological findings to include charophytes in functional trait studies.
Article in Annals of botany, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
background and scopeCharophytes comprise key benthic macroalgae that play multiple ecological roles in freshwater. Despite this, they are excluded from community-level functional trait research, leading to an underestimation of functional diversity of charophyte-dominated ecosystems. This study aims to develop a standardized protocol for measuring leaf functional traits for charophytes, and to provide a first functional comparison between charophytes and common vascular macrophyte life forms.
methodsFunctional units were identified that perform the leaf function and reflect the modular growth of charophytes (whorls, branchlets and internodes). Leaf (branchlet) area, specific leaf area and leaf dry matter content, along with chlorophyll, carotenoid, phosphorus, nitrogen and carbon contents were measured on nine charophyte species. The protocol was validated by comparing traits between charophytes and five macrophyte groups (lemnids, nymphaeids, submerged-broad-leaf, submerged-narrow-leaf and utricularids), using a multidimensional niche approach. KEY
resultsTrait measurements were comparable between charophytes and vascular macrophytes. Charophytes' functional traits showed alignment with ecological expectations, such as increased specific leaf area and reduced dry matter content along the expected growth depth. Overall, charophytes showed high intraspecific variability, attributable to their ability to grow across wide gradients of depth and light. Charophyte functional niche overlapped mostly with submerged narrow-leaved plants but maintained >40 % niche uniqueness.
conclusionsCharophytes expand the functional space occupied by macrophytes, contributing to understanding the overall functional diversity of aquatic plants. Following research should apply the protocol to all genera of the family Characeae and investigate the environmental drivers of trait variability.
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