Evidence map›Paper›PMID 41990243›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Optimization of SWCNT-FET Biosensors by Aptamer Engineering and Toehold-Mediated Strand Displacement.

Haosen Miao, Tingting Zheng, Houlin Yu, Gririraj Manoharan, Gustavo Sant'Anna, Nicholas Bedfrod, Jorge L Chávez, Chang-Seuk Lee, Matteo Palma

Abstract read
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In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Haosen MiaoDepartment of Chemistry, Queen Mary University of London, London, UK.
Tingting ZhengDepartment of Chemistry, Queen Mary University of London, London, UK.
Houlin YuDepartment of Chemistry, Queen Mary University of London, London, UK.
Gririraj ManoharanDepartment of Chemistry, Queen Mary University of London, London, UK.
Gustavo Sant'AnnaSchool of Chemical Engineering, University of New South Wales, Sydney, New South Wales, Australia.
Nicholas BedfrodSchool of Chemical Engineering, University of New South Wales, Sydney, New South Wales, Australia.
Jorge L Chávez711th Human Performance Wing, Air Force Research Laboratory, Ohio, USA.
Chang-Seuk LeeDepartment of Chemistry, Queen Mary University of London, London, UK.ORCID https://orcid.org/0000-0002-5905-800X
Matteo PalmaDepartment of Chemistry, Queen Mary University of London, London, UK.ORCID https://orcid.org/0000-0001-8715-4034

Funding

Institute of Information & Communications Technology Planning & EvaluationKorea government RS-2025-00523684National Research Foundation of KoreaU.S. Air Force Office of Scientific Research FA8655-21-1-7003
6 · The paper itself

Abstract

Aptamer-based single-walled carbon nanotube (SWCNT) field-effect transistor (FET) biosensors provide a compact, label-free, and sensitive platform for real-time biomarker detection. To enhance device performance, we employed a truncated DNA aptamer (CSS.3_cut3) with improved conformational switching upon cortisol binding, boosting the sensitivity of SWCNT-FET biosensors. We further optimized the functionalization using a toehold-mediated strand displacement (TMSD) strategy to dynamically integrate the aptamer onto SWCNTs in solution, simplifying the fabrication process and increasing the sensitivity. Interestingly, introducing a partially complementary protection strand during TMSD assembly resulted in bistable, two-level signal outputs upon cortisol sensing, contrasting with the gradual response of unprotected sensors. This effect likely stems from the competition between aptamer-target binding and residual hybridization, modulating signal dynamics via tuneable electrostatic gating. These results underscore the promise of aptamer engineering and DNA nanotechnology for developing high-performance biosensors with programmable behavior, offering new avenues for real-time sensing, dynamic molecular recognition, and integrated diagnostic technologies.

Indexed as

Aptamers, NucleotideBiosensing TechniquesHydrocortisoneNanotechnologyNanotubes, CarbonTransistors, ElectronicAptamers, NucleotideHydrocortisoneNanotubes, Carbonaptamerbiosensorscortisolsingle‐walled carbon nanotubestoehold‐mediated strand displacement

Identifiers

PMID41990243
PMCPMC13335490

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.