Protocol for isolating stromal cells from lymphoid tissue for performing scRNA-seq.

Spyridon Makris, Daniel Shewring, Agnesska C Benjamin, Sophie E Acton

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Spyridon MakrisStromal Immunology Group, Laboratory for Molecular Cell Biology, University College London, Gower Street, WC1E 6BT London, UK. Electronic address: spyridon.makris.10@ucl.ac.uk.

Daniel ShewringStromal Immunology Group, Laboratory for Molecular Cell Biology, University College London, Gower Street, WC1E 6BT London, UK.

Agnesska C BenjaminStromal Immunology Group, Laboratory for Molecular Cell Biology, University College London, Gower Street, WC1E 6BT London, UK.

Sophie E ActonStromal Immunology Group, Laboratory for Molecular Cell Biology, University College London, Gower Street, WC1E 6BT London, UK. Electronic address: s.acton@ucl.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Stromal cells constitute less than 2% of cells in lymph nodes and are technically difficult to isolate and analyze. Here, we present a protocol to purify fibroblasts from lymphoid tissues in preparation for single-cell RNA sequencing (scRNA-seq) analysis. We describe steps for lymph node dissection and digestion, cell staining, and magnetic separation and also detailed procedures for checking purity and viability. This protocol can be applied to other tissues, including tumors. For complete details on the use and execution of this protocol, please refer to Makris et al.

Indexed as

Cell SeparationLymphoid TissueSequence Analysis, RNASingle-Cell AnalysisStromal CellsAnimalsFibroblastsHumansLymph NodesSingle-Cell Gene Expression AnalysisCell isolationCell separation/fractionationImmunologySingle Cell

Identifiers

PMID41989919

DOI10.1016/j.xpro.2026.104501

PMCPMC13101284

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.