Evidence map›Paper›PMID 41989515›Full record

ArticleJournal of molecular histology2026

Nell-1 inhibits osteoclast differentiation and bone resorption in apical periodontitis.

Chaoran Liang, Zhiyan Zhou, Mengyin Luan, Yatong Li, Jingjing Zong, Qiang Wang, Xiaoying Wang

Abstract read
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Article in Journal of molecular histology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Chaoran Liang *Department of Endodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University and Shandong Key Laboratory of Oral Tissue Regeneration and Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration and Shandong Provincial Clinical Research Center for Oral Diseases, 44-1 Wenhuaxi Road, Jinan, 250012, Shandong, China.
Zhiyan Zhou *Department of Endodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University and Shandong Key Laboratory of Oral Tissue Regeneration and Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration and Shandong Provincial Clinical Research Center for Oral Diseases, 44-1 Wenhuaxi Road, Jinan, 250012, Shandong, China.
Mengyin LuanDepartment of Endodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University and Shandong Key Laboratory of Oral Tissue Regeneration and Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration and Shandong Provincial Clinical Research Center for Oral Diseases, 44-1 Wenhuaxi Road, Jinan, 250012, Shandong, China.
Yatong LiDepartment of Endodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University and Shandong Key Laboratory of Oral Tissue Regeneration and Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration and Shandong Provincial Clinical Research Center for Oral Diseases, 44-1 Wenhuaxi Road, Jinan, 250012, Shandong, China.
Jingjing ZongDepartment of Endodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University and Shandong Key Laboratory of Oral Tissue Regeneration and Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration and Shandong Provincial Clinical Research Center for Oral Diseases, 44-1 Wenhuaxi Road, Jinan, 250012, Shandong, China.
Qiang WangJinan Key Medical and Health Laboratory of Oral Diseases and Tissue Regeneration, Shandong Provincial Key Medical and Health Laboratory of Oral Diseases and Tissue Regeneration, Jinan Stomatological Hospital, Jinan, 250001, Shandong Province, China. wangqiang126email@126.com.
Xiaoying WangDepartment of Endodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University and Shandong Key Laboratory of Oral Tissue Regeneration and Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration and Shandong Provincial Clinical Research Center for Oral Diseases, 44-1 Wenhuaxi Road, Jinan, 250012, Shandong, China. xiaoyingwang@sdu.edu.cn.

Funding

2021 Jinan Municipal Health Commission Science and Technology Plan Project No. 2021-1-422022 Dean's Research Assistance Fund Project No. 2022-02Shandong Natural Science Foundation of China grant no. ZR2019MH014Shandong Natural Science Foundation of China ZR2023QH447
6 · The paper itself

Abstract

Apical periodontitis (AP) is characterized by progressive osteoclast-mediated bone destruction. Although Nell-1 may inhibit osteoclast differentiation, the underlying mechanisms and its specific role in AP remain unclear. This study aims to determine the inhibitory effects of Nell-1 on osteoclast differentiation and bone resorption in apical periodontitis. It further explores whether Nell-1 exerts its function through activation of the Wnt/β-catenin signaling pathway. RAW264.7 cells were transfected with Nell-1 to evaluate osteoclast differentiation through TRAP staining, qRT-PCR, and Western blotting. A rat model of apical periodontitis was established, followed by treatment with Nell-1 protein and the Wnt inhibitor IWR-1. The effects on bone resorption were assessed using micro-CT, histology, and TRAP staining. TRAP staining of RAW264.7 cells demonstrated that Nell-1 overexpression inhibited osteoclastogenesis. The effects were reversed by inhibiting the Wnt pathway with IWR-1. Nell-1 was found to substantially reduce bone resorption and the differentiation of osteoclasts in vivo, according to micro-CT and histological evaluations. By activating the Wnt pathway, Nell-1 effectively prevents osteoclast differentiation and bone resorption in apical periodontitis. The findings suggest that Nell-1 may serve as an adjunctive strategy for apical periodontitis.

Indexed as

Bone ResorptionCalcium-Binding ProteinsCell DifferentiationOsteoclastsPeriapical PeriodontitisAnimalsMaleMiceNerve Tissue ProteinsOsteogenesisRatsRats, Sprague-DawleyRAW 264.7 CellsWnt Signaling PathwayCalcium-Binding ProteinsNell1 protein, ratNerve Tissue ProteinsApical periodontitisBone resorptionNell-1OsteoclastogenesisWnt/β-catenin

Identifiers

PMID41989515

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.