ArticleMicrobiology spectrum2026
Development of a stable low-copy mini F plasmid derivative for evaluating β-lactamase substrate specificity through antimicrobial susceptibility testing.
Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Traditionally, the impact of the substrate specificity of β-lactamase produced by bacteria on β-lactam resistance (β-lactamase-mediated resistance) has been assessed by performing antimicrobial susceptibility testing (AST) on transformants carrying the cloned gene on arbitrarily chosen β-lactamase-free plasmid vectors. However, because the promoter strength and plasmid copy number of these vectors are not standardized, the resulting β-lactamase expression levels vary, further hampering cross-study comparisons. We constructed pMiniF-1 by inserting a
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