Evidence map›Paper›PMID 41988683›Full record

ArticleAdvanced materials (Deerfield Beach, Fla.)2026

Plasmonic Enhancement of Fluorescence and Protein Dynamics in Living Mammalian Cells.

Marco Locarno, Qiangrui Dong, Marco Post, Xin Meng, Cristiano Glessi, Nynke Marije Hettema, Nidas Brandsma, Sebbe Blokhuizen, Alejandro Castañeda Garcia, Srividya Ganapathy and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced materials (Deerfield Beach, Fla.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Marco LocarnoDepartment of Imaging Physics, Delft University of Technology, Delft, The Netherlands.
Qiangrui DongDepartment of Imaging Physics, Delft University of Technology, Delft, The Netherlands.
Marco PostDepartment of Imaging Physics, Delft University of Technology, Delft, The Netherlands.
Xin MengDepartment of Imaging Physics, Delft University of Technology, Delft, The Netherlands.
Cristiano GlessiDepartment of Imaging Physics, Delft University of Technology, Delft, The Netherlands.
Nynke Marije HettemaDepartment of Bionanoscience, Delft University of Technology, Delft, The Netherlands.
Nidas BrandsmaDepartment of Imaging Physics, Delft University of Technology, Delft, The Netherlands.
Sebbe BlokhuizenDepartment of Imaging Physics, Delft University of Technology, Delft, The Netherlands.
Alejandro Castañeda GarciaDepartment of Imaging Physics, Delft University of Technology, Delft, The Netherlands.
Srividya GanapathyDepartment of Imaging Physics, Delft University of Technology, Delft, The Netherlands.
Thieme SchmidtDepartment of Imaging Physics, Delft University of Technology, Delft, The Netherlands.
Lars van RoemburgDepartment of Imaging Physics, Delft University of Technology, Delft, The Netherlands.
Bing XuDepartment of Radiation Science and Technology, Delft University of Technology, Delft, The Netherlands.
Chun-Ting ChoDepartment of Radiation Science and Technology, Delft University of Technology, Delft, The Netherlands.
Liedewij LaanDepartment of Bionanoscience, Delft University of Technology, Delft, The Netherlands.
Miao-Ping ChienDepartment of Molecular Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands.
Daan BrinksDepartment of Imaging Physics, Delft University of Technology, Delft, The Netherlands.ORCID 0000-0002-5550-5140

Funding

Convergence Health and Technology IntegrativeNeuromedicineDelt University of Technology AI Initiative BIOlabHORIZON EUROPE European Research Council 850818- MultivisionNederlandse Organisatie voor Wetenschappelijk Onderzoek 740.018.018Nederlandse Organisatie voor Wetenschappelijk Onderzoek OCENW.XS2.033
6 · The paper itself

Abstract

This study shows that coupling to designed plasmonic nanoparticles can modulate the electrophysiological function of proteins in living mammalian cells. Nanostar-shaped particles, that are robust to biological noise, are designed to enable near-field-coupling to plasma membrane-localized mutated Archaerhodopsin proteins in live cells. The coupled rhodopsins exhibit enhanced fluorescence and an increased response speed to membrane voltage. Incorporating this plasmonic enhancement into a Markov chain photocycle model of the Archaerhodopsin mutant QuasAr6a, shows an increased fluorescence emission rate and manipulation of the protein dynamics through a combination of photocycle transition rate enhancements. The results show an improvement in fluorescence and voltage-response dynamics of the functional QuasAr6a Archaerhodopsin mutant, beyond what has been achievable through genetic engineering. This opens up possibilities for engineering the biological functionality of proteins through plasmonics: manipulating protein photocycles could improve light sensitivity, change optogenetic applications, and lead to fluorescent biosensors with enhanced dynamics.

Indexed as

Archaeal ProteinsAnimalsFluorescenceHEK293 CellsHumansArchaeal Proteinsfluorescencegenetically encoded voltage indicatorslive cellsphotocycleplasmonicsproteinsvoltage imaging

Identifiers

PMID41988683
PMCPMC13472017

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.