Evidence map›Paper›PMID 41987061›Full record

ArticleBMC plant biology2026

Integrated transcriptomic and metabolomic analysis of prohexadione calcium-mediated alleviation of salt stress in oilseed rape (Brassica napus L.) seedlings.

Wenji Zhong, Peng Deng, Naijie Feng, Dianfeng Zheng, Youwei Du, Rui Deng, Zhiyuan Sun, Shaobiao Duan, Xixi Yang, Nana Yue

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Wenji Zhong *College of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.
Peng Deng *College of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.
Naijie FengCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang, 524088, China. fengnj@gdou.edu.cn.
Dianfeng ZhengCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang, 524088, China. zhengdf@gdou.edu.cn.
Youwei DuCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.
Rui DengCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.
Zhiyuan SunCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.
Shaobiao DuanCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.
Xixi YangCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.
Nana YueCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.

Funding

Coastal Agricultural Engineering Technology Research Center of Guangdong Ocean University 230420020Department of Agriculture and Rural Affairs of Guangdong Province 2024KJ31Innovation Team Project for General Colleges and Universities of Guangdong Provincial Department of Education 2021KCXTD011
6 · The paper itself

Abstract

backgroundSalt stress severely limits the growth of oilseed rape (Brassica napus L.). Prohexadione calcium (Pro-Ca) has been reported to alleviate salt stress in oilseed rape. However, the underlying mechanisms of Pro-Ca-mediated salt stress alleviation in oilseed rape remains unclear. In this study, experimental materials were the kale-type oilseed rape varieties moderately salt-tolerant ‘Huayouza 158R’ and highly salt-tolerant ‘Huayouza 62’. 0, 100, and 150 mM NaCl were applied before sowing, and samples were collected after 21 days of cultivation to evaluate Pro-Ca priming on morphological, physiological, and molecular responses.

resultsPro-Ca priming with 100 mM NaCl (Pro-Ca + S100) or 150 mM NaCl (Pro-Ca + S150) regulating morphogenesis, improving photosynthetic efficiency, and enhancing the antioxidant system compared with 100 mM NaCl (S100) and 150 mM NaCl (S150) alone. Transcriptomic analysis identified 381 and 832 differentially expressed genes (DEGs) in Huayouza 158R under Pro-Ca + S100-vs-S100 and Pro-Ca + S150-vs-S150, respectively, and 317 and 184 DEGs in Huayouza 62 under the same. Metabolomic analysis identified 312 and 487 differential metabolites (DIMs) in Huayouza 158R under Pro-Ca + S100-vs-S100 and Pro-Ca + S150-vs-S150, respectively, and 603 and 593 DIMs in Huayouza 62 under the same. Integrated transcriptomic and metabolomic analyses revealed that Pro-Ca priming up-regulated PLC4-related genes in the Huayouza 158R phosphatidylinositol signaling system pathway (ko04070) in S100. Under S150 treatment, Pro-Ca priming up-regulated YUCCA8 and YUCCA9 genes in the Huayouza 158R tryptophan metabolic pathway (ko00380), and down-regulated the abundance of indole-3-acetaldehyde. In Huayouza 62, Pro-Ca priming down-regulated lipoxygenase-related genes in the linoleic acid metabolic pathway (ko00591). In this pathway, lecithin was up-regulated while 9-KODE and 9,10,13-TriHOME were down-regulated in S100. In contrast, in S150, gamma-linolenic acid was up-regulated, while 9-KODE and 9,10,13-TriHOME were down-regulated.

conclusionThe results showed that Pro-Ca priming could alleviate the effects of salt stress in both oilseed rape varieties by regulating morphogenesis, improving photosynthetic efficiency, and enhancing the antioxidant system. However, the specific molecular mechanisms differ between the two varieties. This study provides a molecular basis for the alleviation of salt stress-induced damage by Pro-Ca-mediated in oilseed rape.

Indexed as

Brassica napusSalt StressTranscriptomeGene Expression ProfilingGene Expression Regulation, PlantMetabolomicsSalt ToleranceSeedlingsSodium ChlorideSodium ChlorideMetabolomicsOilseed rapeProhexadione calciumSalt stressTranscriptomics

Identifiers

PMID41987061
PMCPMC13196188

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.