ArticleMethods in molecular biology (Clifton, N.J.)2026
Advances in Cryogenic Techniques for the In Vitro Preservation of Somatic Embryos and Shoot Tips of Plants.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This chapter describes advances in cryopreservation techniques for the safe long-term storage of somatic embryos and shoot apical meristems of different plant species. Several examples are presented that illustrate the progress of vitrification-based procedures, analyzing, from a practical perspective, the modifications incorporated to better adapt cryogenic protocols to these organized tissues.Cryopreservation of somatic embryos ensures the preservation of genetically stable and totipotent embryogenic cultures, essential for large-scale propagation, breeding programs, and the rescue of elite genotypes. Likewise, cryopreservation of shoot apical meristems plays a fundamental role in maintaining pathogen-free plants, protecting rare and endangered species, and preserving plants with high commercial and agricultural value. Therefore, cryopreservation of somatic embryos and shoot apical meristems constitutes an important biotechnological strategy.
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