Evidence map›Paper›PMID 41986757›Full record

ArticleApplied microbiology and biotechnology2026

Development of a double-antibody sandwich ELISA for detecting Peste des petits ruminants virus infection.

Haoyue Xu, Xueliang Zhu, Qian Li, Yuxi Liu, Qi Wang, Xuelian Meng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Haoyue XuState Key Laboratory for Animal Disease Control and Prevention/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Xueliang ZhuState Key Laboratory for Animal Disease Control and Prevention/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Qian LiState Key Laboratory for Animal Disease Control and Prevention/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Yuxi LiuState Key Laboratory for Animal Disease Control and Prevention/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Qi WangState Key Laboratory for Animal Disease Control and Prevention/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Xuelian MengState Key Laboratory for Animal Disease Control and Prevention/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. mengxuelian@caas.cn.

Funding

Lanzhou City Talent Innovation and Entrepreneurship Project 2022-RC-22National Key Research and Development Program of China 2025YFD1800101
6 · The paper itself

Abstract

Peste des petits ruminants (PPR) is an acute, highly contagious viral disease caused by Peste des petits ruminants virus (PPRV). PPR has inflicted severe economic losses on livestock industries across numerous countries in Africa, Asia, and Europe, posing a significant threat to the survival of endangered wild ruminant species. In this study, we performed prokaryotic expression of the PPRV nucleocapsid (N) protein and prepared rabbit-derived polyclonal antibodies (pAbs). We also identified the characteristics of two mouse-derived monoclonal antibodies (mAbs) against the PPRV N protein. The selected mAb was labeled with horseradish peroxidase (HRP). A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established to detect PPRV antigen using rabbit-derived pAbs and HRP-conjugated mAbs. The threshold for the DAS-ELISA was determined to be 0.227. The DAS-ELISA demonstrated minimum detection limits of 0.195 μg/mL for the recombinant PPRV N protein and 250 50% tissue culture infectious dose (TCID

Indexed as

Antibodies, ViralPeste-des-Petits-RuminantsPeste-des-petits-ruminants virusAnimalsAntibodies, MonoclonalAntigens, ViralEnzyme-Linked Immunosorbent AssayGoatsMiceNucleocapsid ProteinsRabbitsSensitivity and SpecificitySheepAntibodies, MonoclonalAntibodies, ViralAntigens, ViralNucleocapsid ProteinsDouble antibody sandwich ELISAMonoclonal antibodyNucleocapsid proteinPeste des petits ruminantsPolyclonal antibody

Identifiers

PMID41986757
PMCPMC13201319

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.