Evidence map›Paper›PMID 41986708›Full record

ArticleNature2026

Molecular basis for methylation-sensitive editing by Cas9.

Mitchell O Roth, Yuerong Shu, Yu Zhao, Despoina Trasanidou, Renee D Hoffman, Christian Südfeld, Eugenios Bouzetos, Nikolaos Trasanidis, Michael Zawrotny, Mary K Gelasco and 7 more

Abstract read
In one paragraph

Article in Nature, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Mitchell O Roth *Department of Structural Biology, Van Andel Institute, Grand Rapids, MI, USA.
Yuerong Shu *Department of Structural Biology, Van Andel Institute, Grand Rapids, MI, USA.
Yu Zhao *Department of Structural Biology, Van Andel Institute, Grand Rapids, MI, USA.ORCID http://orcid.org/0000-0003-0485-2780
Despoina Trasanidou *Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Wageningen, Netherlands.
Renee D Hoffman *Graduate School, Van Andel Institute, Grand Rapids, MI, USA.
Christian SüdfeldLaboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Wageningen, Netherlands.
Eugenios BouzetosLaboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Wageningen, Netherlands.
Nikolaos TrasanidisHugh & Josseline Langmuir Centre for Myeloma Research, Centre for Hematology, Department of Immunology and Inflammation, Imperial College London, London, UK.ORCID http://orcid.org/0000-0001-9795-3059
Michael ZawrotnyInstitute of Molecular Biophysics, Tallahassee, FL, USA.ORCID http://orcid.org/0000-0003-3830-9321
Mary K GelascoDepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, FL, USA.
Megan L MedinaDepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, FL, USA.
Anuska DasInstitute of Molecular Biophysics, Tallahassee, FL, USA.
Jay RaiInstitute of Molecular Biophysics, Tallahassee, FL, USA.
Hemant N GoswamiDepartment of Structural Biology, Van Andel Institute, Grand Rapids, MI, USA.
Bing WangDepartment of Structural Biology, Van Andel Institute, Grand Rapids, MI, USA.
John van der OostLaboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Wageningen, Netherlands. john.vanderoost@wur.nl.ORCID http://orcid.org/0000-0001-5024-1871
Hong LiDepartment of Structural Biology, Van Andel Institute, Grand Rapids, MI, USA. hong.li@vai.org.ORCID http://orcid.org/0000-0003-2046-9861

Funding

Cancer Epigenetics Training (CET) ProgramT32CA251066 · NCI · VAN ANDEL RESEARCH INSTITUTE · PI Scott Rothbart · 2021 to 2026
$2.4M
Mechanisms and Applications of RNA-mediated Enzymes in Translation and ImmunityR35GM152081 · NIGMS · VAN ANDEL RESEARCH INSTITUTE · PI Hong Li · 2024 to 2026
$1.8M
NCI NIH HHS T32 CA251066NIGMS NIH HHS R35 GM152081
6 · The paper itself

Abstract

The bacterial CRISPR-Cas9 (Cas9) nuclease has become a powerful genome manipulation tool for a wide range of organisms

Indexed as

CRISPR-Cas SystemsDNA MethylationGene EditingCRISPR-Associated Protein 9CRISPR-Associated ProteinsCryoelectron MicroscopyCytosineDNADNA CleavageFemaleGenome, HumanHumansModels, MolecularNucleotide MotifsCRISPR-Associated Protein 9CRISPR-Associated ProteinsCytosineDNA

Identifiers

PMID41986708
PMCPMC13216068

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.