ArticleNature methods2026
A series of spontaneously blinking dyes for super-resolution microscopy.
Article in Nature methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- Self-Blinking Dye Restores Efficient Use of Nanobodies in Single-Molecule Localization Microscopy.Nano letters · 2026Article
- Functional differences of Munc18 isoforms reflect their affinities for the exocytosis site.Cellular and molecular life sciences : CMLS · 2026Article
- Single-Step Expansion SMLM Enables Molecular-Resolution Imaging in Cells and Isolated Proteins.Chemical & biomedical imaging · 2026Article
- Brightness demixing for simultaneous multi-target imaging in 3D single-molecule localization microscopy.Nature methods · 2026Article
- Optical single-channel recording of CRAC channels with HaloTag and a CabioRxiv : the preprint server for biology · 2026Article
- Single-Molecule Fluorescence Defines a New Dye Chemistry.Chemical & biomedical imaging · 2026Review
- A single-molecule reporter of membrane-proximal actin detects rapid remodeling upon B cell receptor clustering.bioRxiv : the preprint server for biology · 2026Article
- FLEXTAG: a small and self-renewable protein labeling system for anti-fading multi-color super-resolution imaging.Nature communications · 2026Article
- Review of expansion microscopy combined with advanced imaging modalities.Journal of microscopy · 2026Review
- Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies.Chemical & biomedical imaging · 2025Review
- Clustering within a single-component biomolecular condensate.bioRxiv : the preprint server for biology · 2025Article
- FLEXTAG: A Small and Self-renewable Protein Labeling System for Anti-fading Multicolor Super-resolution Imaging.bioRxiv : the preprint server for biology · 2025Article
- Mapping the nuclear landscape with multiplexed super-resolution fluorescence microscopy.Nature communications · 2025Article
- Experimental Approaches to Visualize Effector Protein Translocation During Host-Pathogen Interactions.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals.Nature communications · 2024Article
- Robust Quantification of Live-Cell Single-Molecule Tracking Data for Fluorophores with Different Photophysical Properties.The journal of physical chemistry. B · 2024Article
- Correlative single molecule lattice light sheet imaging reveals the dynamic relationship between nucleosomes and the local chromatin environment.Nature communications · 2024Article
Corrections and comments
- Update of
Authors and funding
21 authors.
Funding
Abstract
Spontaneously blinking fluorophores toggle between nonfluorescent and fluorescent forms without caging groups or redox buffers, enabling super-resolution imaging. The intrinsic blinking of such dyes is governed by molecular structure and modulated by environment; there is no one-size-fits-all fluorophore suitable for every imaging context. We report dyes with tuned on:off ratios that enable single-molecule localization microscopy and super-resolution optical fluctuation imaging of biomolecular structures in vitro and in cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.