ArticleScientific reports2026
Method comparison of microscopy, metabarcoding, and multispectral imaging flow cytometry for identification and relative abundance analysis of insect-dispersed pollen.
Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Pollen identification and quantification are essential in ecological and evolutionary research to address plant-pollinator relationships, pollination services, and plant reproduction. Research into pollen transfer patterns has been mainly based on traditional light microscopy, however, it is time consuming, labour intensive, and requires taxonomic expertise. High-throughput methods allow automated pollen identification across large temporal and spatial scales. This study compares the accuracy of species identification and quantification of their relative pollen abundance using traditional microscopy, and two high-throughput methods, namely multispectral imaging flow cytometry (MIFC) and metabarcoding. Method performance was tested using artificial samples with known pollen species composition and pollen samples from pollinators with unknown pollen composition. After checking the agreement against the line of identity, the coefficient of determination (R
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