Evidence map›Paper›PMID 41985841›Full record

ArticleJournal of lipid research2026

Mammarenaviruses depend on endogenous fatty acid synthesis in cell culture systems.

Joseph Thomas Noble, Maryam Siddique, Kingsley Bimpeh, Nathaniel Jackson, Amaani Ibrahim, Thomas Lucien McDaniel, Nathan W Davis, Caitlin Bone Wade, Luis Martínez-Sobrido, Kelly Marie Hines and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of lipid research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Joseph Thomas NobleDepartment of Infectious Diseases, University of Georgia, Athens, GA, USA.
Maryam SiddiqueDepartment of Infectious Diseases, University of Georgia, Athens, GA, USA.
Kingsley BimpehDepartment of Chemistry, University of Georgia, Athens, GA, USA.
Nathaniel JacksonTexas Biomedical Research Institute, TX, USA.
Amaani IbrahimDepartment of Infectious Diseases, University of Georgia, Athens, GA, USA.
Thomas Lucien McDanielDepartment of Infectious Diseases, University of Georgia, Athens, GA, USA.
Nathan W DavisDepartment of Infectious Diseases, University of Georgia, Athens, GA, USA.
Caitlin Bone WadeDepartment of Infectious Diseases, University of Georgia, Athens, GA, USA.
Luis Martínez-SobridoTexas Biomedical Research Institute, TX, USA.
Kelly Marie HinesDepartment of Chemistry, University of Georgia, Athens, GA, USA.
Melinda Ann BrindleyDepartment of Infectious Diseases, University of Georgia, Athens, GA, USA; Department of Population Health, University of Georgia, Athens, GA, USA. Electronic address: mbrindle@uga.edu.

Funding

Defining the role of phosphatidylserine in hemorrhagic fever virus replicationR01AI139238 · NIAID · UNIVERSITY OF GEORGIA · PI BRINDLEY, MELINDA ANN · 2019 to 2023
$2.0M
NIAID NIH HHS R01 AI139238
6 · The paper itself

Abstract

Lassa virus (LASV) and lymphocytic choriomeningitis virus (LCMV) are Old World mammarenaviruses that, like all viruses, rely on host-derived biological molecules to complete their replication cycle. Identifying host factors essential for mammarenavirus replication may reveal novel targets for antiviral intervention. To this end, we found that replication of recombinant tri-segmented r3LCMV and r3LCMV LASV chimera (r3LCMV-LASV) was sensitive to reductions in both exogenously supplied and endogenously synthesized lipids. Lipidomic analysis on mock-infected and virus-infected VeroS cells revealed infection with r3LCMV-LASV increased the abundance of triacylglycerols (TG), phosphatidylcholine (PC), and phosphatidylglycerol (PG), while decreasing levels of ceramides, phosphatidylethanolamine (PE), and phosphatidylserine (PS). Although TG levels rose during infection, pharmacologic inhibition of TG synthesis did not impair viral replication. In contrast, inhibition of fatty acid synthase (FASN), a key enzyme upstream of TG synthesis, significantly reduced r3LCMV-LASV and r3LCMV spread. FASN inhibition suppressed both viral genome replication and viral budding. The addition of oleic acid, but not palmitic acid (the principal product of FASN), rescued the inhibitory effect of FASN blockade. Reducing FASN using shRNA conferred similar effects. Dependence on FASN activity was observed across multiple New and Old World mammarenaviruses and in multiple different cell lines, further reinforcing the importance of fatty acid synthase for productive mammarenaviral infection.

Indexed as

Fatty AcidsLassa virusAnimalsChlorocebus aethiopsVero CellsVirus ReplicationFatty AcidsFASNfatty acidsLassalipidsmammarenavirus

Identifiers

PMID41985841
PMCPMC13199888

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.