Evidence map›Paper›PMID 41984279›Full record

ArticleAdvanced biotechnology2026

Engineering a robust IsPETase for energy-efficient PET depolymerization in natural seawater at ambient temperatures.

Xin Huang, Qian Jia, Guang Li, Xiangpeng Yang, Shujing Xu, Jianzhong Liu, Wenjun Li, Yuhuan Liu, Wei Xie, Lichuang Cao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xin HuangState Key Laboratory of Biocontrol, Innovation Center for Evolutionary Synthetic Biology, Guangzhou Innovation Center of Biotechnology and Biomanufacturing, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
Qian JiaState Key Laboratory of Biocontrol, Innovation Center for Evolutionary Synthetic Biology, Guangzhou Innovation Center of Biotechnology and Biomanufacturing, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
Guang LiState Key Laboratory of Biocontrol, Innovation Center for Evolutionary Synthetic Biology, Guangzhou Innovation Center of Biotechnology and Biomanufacturing, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.ORCID http://orcid.org/0000-0002-3077-5929
Xiangpeng YangState Key Laboratory of Biocontrol, Innovation Center for Evolutionary Synthetic Biology, Guangzhou Innovation Center of Biotechnology and Biomanufacturing, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.ORCID http://orcid.org/0000-0001-7009-1532
Shujing XuState Key Laboratory of Biocontrol, Innovation Center for Evolutionary Synthetic Biology, Guangzhou Innovation Center of Biotechnology and Biomanufacturing, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
Jianzhong LiuState Key Laboratory of Biocontrol, Innovation Center for Evolutionary Synthetic Biology, Guangzhou Innovation Center of Biotechnology and Biomanufacturing, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
Wenjun LiState Key Laboratory of Biocontrol, Innovation Center for Evolutionary Synthetic Biology, Guangzhou Innovation Center of Biotechnology and Biomanufacturing, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
Yuhuan LiuState Key Laboratory of Biocontrol, Innovation Center for Evolutionary Synthetic Biology, Guangzhou Innovation Center of Biotechnology and Biomanufacturing, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.ORCID http://orcid.org/0000-0002-6184-3760
Wei XieState Key Laboratory of Biocontrol, Innovation Center for Evolutionary Synthetic Biology, Guangzhou Innovation Center of Biotechnology and Biomanufacturing, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China. xiewei6@mail.sysu.edu.cn.
Lichuang CaoState Key Laboratory of Biocontrol, Innovation Center for Evolutionary Synthetic Biology, Guangzhou Innovation Center of Biotechnology and Biomanufacturing, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China. caolch5@mail.sysu.edu.cn.ORCID http://orcid.org/0000-0002-0996-8419

Funding

Guangdong S&T Program 2022B1111050002Guangdong S&T Program 2024B1111130003Key Research Program Project of Guangzhou Science and Technology Bureau 2024B03J1276National Key Research and Development Program of China 2024YFC3407000National Natural Science Foundation of China 31870782National Natural Science Foundation of China 32000031National Natural Science Foundation of China 91951205National Natural Science Foundation of China 91951207
6 · The paper itself

Abstract

Enzymatic depolymerization of polyethylene terephthalate (PET), the world's most widely used polyester, in seawater at ambient temperature offers a promising and energy-efficient route for freshwater-free plastic recycling. While a number of PET hydrolases have been reported in recent years, their potential under saline conditions remains largely unexplored. Here, we screened eight enzymes in artificial seawater at 30 °C and engineered the most active one, IsPETase, using a semi-rational strategy focused on rigidifying flexible sites. The resulting variant M8 showed simultaneous enhancementsin thermostability (ΔT

Indexed as

Bio-recyclingEnzymatic depolymerizationEnzyme engineeringPET hydrolaseSeawater bio-catalysisSimultaneous enzymatic depolymerization and fermentation (SEDF)

Identifiers

PMID41984279
PMCPMC13083688

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.