ArticleMikrochimica acta2026
Sensitive detection of Salmonella enterica subsp. enterica serovar typhimurium based on antibody-aptamer dual recognition and DNA walker signal amplification.
Article in Mikrochimica acta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
A highly sensitive and specific immunoassay for the detection of Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) is presented, which integrates aptamer recognition with a DNA walker-based signal amplification strategy. Target bacteria are first captured by plate-immobilized antibodies and then specifically bound by an aptamer. Free unbound aptamer in the supernatant is harvested and sequesters a small fraction of initiator DNA, leaving most available for hybridization with, fluorescein-labeled thiolated DNA (SH-DNA-FAM). The initiator DNA/SH-DNA-FAM duplex then recruits the nicking endonuclease Nb.BbvCI, which cleaves the SH-DNA-FAM and releases the fluorophore from the AuNP-mediated quenching state. The liberated initiator DNA is recycled to drive successive cleavage events, resulting in a robust DNA walker amplification cascade and a strongly enhanced fluorescence signal. This method achieves a detection limit of 43 CFU/mL with a linear range of 102–106 CFU/mL (R2 = 0.98). Moreover, it not only displays excellent specificity but also enables rapid signal amplification, demonstrating considerable potential for food-safety monitoring.
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