Evidence map›Paper›PMID 41983694›Full record

ArticleReproduction in domestic animals = Zuchthygiene2026

Assessing Long-Term Changes in Endometrial Morphology and Functionality in Dairy Cows With Metritis.

M H Rashid, A Martelo Pereira, F N S Pereira, M C Oliveira Costa, E F Santos, L Christensen, J A Sexton, M Ciccarelli, L B Williams, M Binelli and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproduction in domestic animals = Zuchthygiene, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

M H RashidDepartment of Veterinary Clinical Sciences, Field Disease Investigation Unit, Washington State University, Pullman, Washington, USA.
A Martelo PereiraDepartment of Veterinary Clinical Sciences, Field Disease Investigation Unit, Washington State University, Pullman, Washington, USA.
F N S PereiraDepartment of Veterinary Clinical Sciences, Field Disease Investigation Unit, Washington State University, Pullman, Washington, USA.
M C Oliveira CostaDepartment of Veterinary Clinical Sciences, Field Disease Investigation Unit, Washington State University, Pullman, Washington, USA.
E F SantosDepartment of Veterinary Clinical Sciences, Field Disease Investigation Unit, Washington State University, Pullman, Washington, USA.
L ChristensenDepartment of Animal, Veterinary and Food Sciences, University of Idaho, Moscow, Idaho, USA.
J A SextonDepartment of Veterinary Clinical Sciences, Field Disease Investigation Unit, Washington State University, Pullman, Washington, USA.
M CiccarelliDepartment of Veterinary Clinical Sciences, Field Disease Investigation Unit, Washington State University, Pullman, Washington, USA.
L B WilliamsDepartment of Veterinary Microbiology and Pathology, Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman, Washington, USA.
M BinelliDepartment of Animal Sciences, University of Florida, Gainesville, Florida, USA.
M S WaqasDepartment of Veterinary Clinical Sciences, Field Disease Investigation Unit, Washington State University, Pullman, Washington, USA.
E B de OliveiraZoetis Inc, Parsippany, New Jersey, USA.
C C FigueiredoDepartment of Veterinary Clinical Sciences, Field Disease Investigation Unit, Washington State University, Pullman, Washington, USA.ORCID https://orcid.org/0000-0003-0890-0743

Funding

U.S. Department of Agriculture 7005594
6 · The paper itself

Abstract

This study evaluated the long-term impact of metritis on endometrial morphology and functionality in dairy cows. Postpartum Holstein cows (n = 107) were enrolled in a cohort study at the University of Idaho Dairy Center. Metritis was diagnosed using a Metricheck device within 12 days in milk (DIM) based on the presence of fetid, watery, reddish-brown vaginal discharge (VD). Cows diagnosed with metritis (MET; n = 9) were treated with ceftiofur crystalline free acid and paired with cows without metritis (NoMET; n = 9) of similar parity and DIM. Ovulation was synchronized using a Double Ovsynch protocol starting at 35 DIM, and ovulation was confirmed by ultrasonography. To eliminate confounding effects of semen or conceptus-derived signals, cows were not inseminated. Six days after ovulation (68-75 DIM), endometrial biopsy and cytology samples were collected. Endometrial morphology was assessed by histological evaluation of haematoxylin and eosin-stained sections. Endometrial transcriptomic profiles were determined by RNA sequencing from tissues collected via biopsy, and uterine fluid collected during cytology was analysed using metabolomic profiling. Differential gene expression was assessed using DESeq2, and metabolomic differences were evaluated using partial least squares discriminant analysis and PERMANOVA. Cows with metritis exhibited a numerically greater prevalence of periglandular fibrosis (63%; 95% Exact Binomial Confidence Interval [CI] = 25-92) compared with NoMET cows (44%; 95% CI = 14-79). Transcriptomic analysis identified 17 differentially expressed genes (FDR ≤ 0.10; |log2 fold change| > 1), including upregulation of genes associated with tissue repair in MET cows. No differences were detected in uterine fluid metabolomic profiles between groups. In conclusion, metritis was associated with persistent alterations in endometrial morphology and gene expression that were detectable months after clinical resolution, although these changes were not reflected in the uterine fluid metabolome.

Indexed as

Cattle DiseasesEndometritisEndometriumAnimalsCattleCohort StudiesFemalePostpartum PeriodTranscriptomefertilityMultiOmicsuterus

Identifiers

PMID41983694
PMCPMC13081769

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.