Evidence map›Paper›PMID 41983365›Full record

ArticleSmall (Weinheim an der Bergstrasse, Germany)2026

Multi-Tiered µDicer Enables Protein-Preserving Microdissection at 10 µm Resolution.

Annatoma Arif, Rashmi Kumar, Yumi Kwon, Ramon Rodriguez, Seth Charles Cordts, Saisneha Koppaka, Ying Zhu, Ljiljana Paša-Tolić, Sindy Kam Yan Tang

Abstract read
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In one paragraph

Article in Small (Weinheim an der Bergstrasse, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Annatoma ArifDepartment of Mechanical Engineering, Stanford University, Stanford, California, USA.ORCID https://orcid.org/0000-0003-3264-4700
Rashmi KumarEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA.
Yumi KwonEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA.ORCID https://orcid.org/0000-0003-0523-6197
Ramon RodriguezDepartment of Mechanical Engineering, Stanford University, Stanford, California, USA.
Seth Charles CordtsDepartment of Mechanical Engineering, Stanford University, Stanford, California, USA.
Saisneha KoppakaDepartment of Mechanical Engineering, Stanford University, Stanford, California, USA.
Ying ZhuEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA.
Ljiljana Paša-TolićEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA.
Sindy Kam Yan TangDepartment of Mechanical Engineering, Stanford University, Stanford, California, USA.ORCID https://orcid.org/0000-0003-1465-2432

Funding

An integrated microtechnology platform for spatially resolved mass spectrometry-based proteomicsR01CA283247 · NCI · STANFORD UNIVERSITY · PI Sindy Kam-Yan Tang · 2023 to 2026
$2.4M
A micro-dissection platform for generating uniform-sized patient-derived tumor organoids (PDOs) for personalized cancer therapyR21CA261643 · NCI · STANFORD UNIVERSITY · PI TANG, SINDY KAM-YAN · 2022 to 2024
$573k
Department of Energy DE-AC05-76RL01830National Science Foundation DBI-1548297NCI NIH HHS R01 CA283247NCI NIH HHS R01CA283247NCI NIH HHS R21 CA261643NCI NIH HHS R21CA261643Stanford Bio-X R10-14Stanford Center for Cancer Nanotechnology Excellence for Translational Diagnostics (CCNE-TD)Stanford University Beckman Center Technology Development Seed Grants
6 · The paper itself

Abstract

Laser capture microdissection (LCM) remains the leading technology for isolating sub-millimeter regions of interest (ROIs) to enable downstream molecular profiling and study tissue heterogeneity. As the ROI approaches cellular dimensions (∼10 µm), laser-induced photothermal damage and challenges in capturing microtissues in conventional LCM can compromise protein preservation and quantitative fidelity. This work introduces multi-tiered µDicers, fabricated by two-photon polymerization, to mechanically dissect tissue slices into uniform microtissues down to 10 µm. The hierarchical blade architecture limits instantaneous blade-tissue engagement and lowers the cutting force relative to single-tier designs. For benchmarking, proteomic analysis is performed on ethanol-fixed human squamous cell carcinoma microtissues generated by µDicers and by LCM. Under identical Nanodroplet Processing in One pot for Trace Samples (nanoPOTS) and liquid chromatography-mass spectrometry (LC-MS) conditions, µDicers yield more peptides and proteins than LCM, with the largest gains at 10-20 µm spatial resolution. Confocal imaging shows catapult-associated cavities in LCM-generated microtissues. This material loss, along with membrane-limited protein extraction, likely reduces protein coverage. In contrast, multi-tiered µDicers enable reproducible microdissection down to 10 µm while maintaining high protein coverage. With the spatial registration of microtissues under development, µDicers have the potential to complement LCM for next-generation spatial proteomic workflows.

Indexed as

Laser Capture MicrodissectionMicrodissectionProteinsHumansLiquid Chromatography-Mass SpectrometryProteomicsProteinshierarchical blade arraysmass spectrometryspatial proteomicstissue microdissectiontwo‐photon polymerization

Identifiers

PMID41983365
PMCPMC13351537

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.