Evidence map›Paper›PMID 41982338›Full record

ArticlebioRxiv : the preprint server for biology2026

MAPseq2: a sensitive barcoded connectomics method.

Hyopil Kim, Huihui Qi, Craig Washington, Xiaoping Liang, Justus M Kebschull

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Hyopil KimDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.ORCID 0000-0001-9159-8682
Huihui QiDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Craig WashingtonDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Xiaoping LiangDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Justus M KebschullDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.ORCID 0000-0002-4851-0267

Funding

BRAIN CONNECTS: Mapping brain-wide connectivity of neuronal types using barcoded connectomicsU01NS132161 · NINDS · ALLEN INSTITUTE · PI CHEN, XIAOYIN, KEBSCHULL, JUSTUS M · 2023 to 2025
$6.7M
Cell type specific vulnerability to agingRF1AG078378 · NIA · JOHNS HOPKINS UNIVERSITY · PI PATRICK O KANOLD, JUSTUS M KEBSCHULL · 2023 to 2026
$4.0M
IDENTIFYING EPIGENOMICS AND CONNECTOMICS OF PROTRACTED OPIOID WITHDRAWAL AND RELAPSE USING CELLULAR BARCODINGDP1DA056668 · NIDA · JOHNS HOPKINS UNIVERSITY · PI JUSTUS M KEBSCHULL · 2022 to 2026
$2.5M
NIA NIH HHS RF1 AG078378NIDA NIH HHS DP1 DA056668NINDS NIH HHS U01 NS132161
6 · The paper itself

Abstract

The barcoded connectomics tool MAPseq enables highly multiplexed projection mapping of individual neurons by translating neuroanatomy into a format solvable by DNA sequencing. Here we present MAPseq2, a user-friendly protocol with up to ~10-fold increased sensitivity compared to the current state of the art and ~10-fold decreased cost. As MAPseq workflows are used across a range of barcoded connectomics methods, including BARseq, BRICseq, and ConnectID, all improvements in MAPseq2 directly transfer to these technologies.

Identifiers

PMID41982338
PMCPMC13075454

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.