Evidence map›Paper›PMID 41981648›Full record

ArticleJournal of nanobiotechnology2026

In-situ polymerization-mediated glycan density measurement on extracellular vesicle surface for acute myeloid leukemia diagnosis.

Xingjie Wu, Weifeng Long, Litao Zhang, Cong Luo, Xiaoxia Hu, Ling Tao, Jie Xiong, Xiangchun Shen, Haitao Zhao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Aptamer-functionalized aggregation-induced emission micelles for integrated detection and photodegradation of lipopolysaccharides.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Xingjie Wu *The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province, School of Pharmaceutical Sciences, Guizhou Medical University, No.6 Ankang Avenue, Guiyang, 561113, Guizhou, China. wxj_gmu@126.com.
Weifeng Long *The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province, School of Pharmaceutical Sciences, Guizhou Medical University, No.6 Ankang Avenue, Guiyang, 561113, Guizhou, China.
Litao Zhang *Ministry of Education Key Laboratory of Micro and Nano Systems for Aerospace, School of Mechanical Engineering, Northwestern Polytechnical University, Xi'an, 710072, China.
Cong LuoDepartment of Pharmaceutics, Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, 110016, P. R. China.
Xiaoxia HuThe High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province, School of Pharmaceutical Sciences, Guizhou Medical University, No.6 Ankang Avenue, Guiyang, 561113, Guizhou, China.
Ling TaoThe High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province, School of Pharmaceutical Sciences, Guizhou Medical University, No.6 Ankang Avenue, Guiyang, 561113, Guizhou, China.
Jie XiongDepartment of Hematology, The Affiliated Hospital of Guizhou Medical University, Guiyang, 550004, Guizhou, China. xiongjie716@126.com.
Xiangchun ShenThe High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province, School of Pharmaceutical Sciences, Guizhou Medical University, No.6 Ankang Avenue, Guiyang, 561113, Guizhou, China. shenxiangchun@126.com.
Haitao ZhaoMinistry of Education Key Laboratory of Micro and Nano Systems for Aerospace, School of Mechanical Engineering, Northwestern Polytechnical University, Xi'an, 710072, China. zhaoht@nwpu.edu.cn.

Funding

National Natural Science Foundation of China 22464007
6 · The paper itself

Abstract

Extracellular vesicles (EVs) have emerged as promising circulating biomarkers for diverse pathologies, yet clinical adoption remains limited due to inter-patient variability in blood EV concentrations, which introduces inconsistent biomarker signals and reduces diagnostic reliability. To address this, we developed an in-situ dopamine polymerization method for precise glycan density quantification on EVs expressing a specific membrane protein. EV membrane proteins were labeled using streptavidin-horseradish peroxidase-conjugated aptamers, while surface lipids and glycans were simultaneously tagged with fluorescent cholesterol and lectin, respectively. Controlled dopamine polymerization spatially restricted fluorescence quenching to EV membranes, enabling glycan density calculation via attenuation profiles of cholesterol- and lectin-bound fluorophores. The method was miniaturized into a microfluidic platform for rapid (< 1 h), wash-free point-of-care analysis. In 47 clinical specimens (16 patients with acute myeloid leukemia, 15 patients with benign hematological diseases, and 16 healthy donors), glycan density normalization reduced inter-patient variability compared to absolute measurements. Multi-lectin analysis of CD133

Indexed as

Extracellular VesiclesLeukemia, Myeloid, AcutePolysaccharidesBiomarkers, TumorHumansLectinsPolymerizationBiomarkers, TumorLectinsPolysaccharidesAcute myeloid leukemia diagnosisEV-templated polymerizationGlycan density measurementWash-free EV analyzing tool

Identifiers

PMID41981648
PMCPMC13200366

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.