Effect of heat stress and GlyNAC supplementation on jejunal morphology, hepatic inflammation infiltration and distribution of GSH content in chickens.

Chen Wang, Liya Jin, Zhengshun Jia, Tengfei Ma, Quanwei Wei, Yansen Li

Abstract read

In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Gut microbiota contributes to heat tolerance in chickens through metabolic remodeling and suppression of thermogenesis.Poultry science · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Chen WangResearch Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2024205036@stu.njau.edu.cn.

Liya JinResearch Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2024105061@stu.njau.edu.cn.

Zhengshun JiaResearch Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2023805124@stu.njau.edu.cn.

Tengfei MaResearch Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2024805021@stu.njau.edu.cn.

Quanwei WeiResearch Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; School of Medicine, University of Virginia, Charlottesville 22901, USA. Electronic address: weiquanwei@njau.edu.cn.

Yansen LiResearch Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: liyansen@njau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study evaluated the effects of cyclic heat stress (cHS) and diets supplemented with a combination of glycine and N-acetylcysteine (GlyNAC) on growth performance, serum antioxidant capacity and glutathione (GSH) content in serum, liver and pectoral muscles of Cyan-shank partridge chickens. Sixty-four male chickens (60 days old) were selected and randomly divided into four groups: CON + TN (basal diet; TN, thermoneutral condition with continuous 25 °C), GlyNAC + TN (basal diet + GlyNAC; TN), CON + cHS (basal diet; cHS, 32 °C for 4 h daily), and GlyNAC + cHS (basal diet + GlyNAC; cHS). The experimental period was 7 days. Results indicated that cHS significantly increased rectal temperature of chickens during heat treatment period (P < 0.05). Nevertheless, neither cHS nor GlyNAC supplementation affected growth performance or serum malondialdehyde levels. GlyNAC supplementation increased the GSH content in serum, liver and pectoral muscle (P < 0.05), but neither cHS nor GlyNAC altered jejunal GSH levels. The cHS decreased the ratio of villous height to crypt depth (V:C) and increased hepatic inflammatory infiltration score (IIS), while GlyNAC supplementation improved both the V:C ratio and hepatic IIS (P < 0.05). In liver, cHS downregulated the protein expression of caspase 3 and glutamate-cysteine ligase modifier subunit (GCLM), as well as the mRNA expression of GCLM gene. In contrast, GlyNAC supplementation upregulated hepatic caspase 3 protein expression and the mRNA expression of GCLM genes (P < 0.05). In conclusion, dietary GlyNAC supplementation maintained the elevated GSH content in both serum and liver, while alleviating cHS-induced impairment of jejunal integrity and hepatic inflammatory infiltration.

Indexed as

AcetylcysteineChickensGlutathioneGlycineHeat-Shock ResponseHot TemperatureJejunumAnimal FeedAnimalsAntioxidantsDietDietary SupplementsInflammationLiverMalePectoralis MusclesAcetylcysteineAntioxidantsGlutathioneGlycineCaspase 3GlyNACGSHJejunumPectoral muscle

Identifiers

PMID41980553

DOI10.1016/j.psj.2026.106910

PMCPMC13092076

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.