Activation of Escherichia coli RecA* in recA730 lexA

John P McDonald, Jaan C Selod, Marcela Teatin Latancia, Roger Woodgate

Abstract read

In one paragraph

Article in DNA repair, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

John P McDonaldLaboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-3371, USA.

Jaan C SelodLaboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-3371, USA.

Marcela Teatin LatanciaLaboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-3371, USA.

Roger WoodgateLaboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-3371, USA. Electronic address: woodgate@nih.gov.

Funding

DNA Replication, Repair, and Mutagenesis In Eukaryotic And Prokaryotic CellsZIAHD001500 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI WOODGATE, ROGER · 2009 to 2025

$37.5M

Intramural NIH HHS ZIA HD001500

6 · The paper itself

Abstract

The Escherichia coli RecA protein plays central roles in mediating homologous recombination, induction of the damage inducible SOS response and cellular mutagenesis. All three roles require that monomeric RecA assembles on single-stranded DNA to form a RecA-nucleoprotein filament that is often referred to as "activated RecA" or RecA*. Wild-type RecA normally forms RecA* after DNA damage. However, certain missense substitutions in RecA enable the protein to form RecA* spontaneously in the absence of DNA damage. One such allele is recA730 (E38K) which has historically been thought to be "constitutively activated". Based upon quantitative western blot analysis of several SOS-regulated proteins (RecA, LexA, UmuD', UmuC and DinB), we discovered that RecA730 is only partially activated in a lexA

Indexed as

Bacterial ProteinsEscherichia coliEscherichia coli ProteinsRec A RecombinasesSerine EndopeptidasesDNA, BacterialDNA-Binding ProteinsDNA DamageLexA Repressor ProteinSOS Response, GeneticsBacterial ProteinsDNA, BacterialDNA-Binding ProteinsEscherichia coli ProteinsLexA Repressor ProteinrecA protein, E coliRec A RecombinasesSerine EndopeptidasesDinBDNA polymerase VLexARecARNA polymerase αSOS response

Identifiers

PMID41980523

DOI10.1016/j.dnarep.2026.103933

PMCPMC13080208

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.