Evidence map›Paper›PMID 41979903›Full record

ArticleACS synthetic biology2026

PHYCUT: Scalable Multiplex CRISPR/Cas9 Editing for Genome Engineering in the Diatom

Emily E Stuckless, Lian S Gai, Samuel S Slattery, Kira H Dempsey, Tyler S Browne, Gregory B Gloor, David R Edgell

Abstract read
In one paragraph

Article in ACS synthetic biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Emily E StucklessDepartment of Biochemistry, Schulich School of Medicine & Dentistry, Western University, London ON N6A 5C1, Canada.
Lian S GaiDepartment of Biochemistry, Schulich School of Medicine & Dentistry, Western University, London ON N6A 5C1, Canada.
Samuel S SlatteryDepartment of Biochemistry, Schulich School of Medicine & Dentistry, Western University, London ON N6A 5C1, Canada.
Kira H DempseyDepartment of Biochemistry, Schulich School of Medicine & Dentistry, Western University, London ON N6A 5C1, Canada.
Tyler S BrowneDepartment of Biochemistry, Schulich School of Medicine & Dentistry, Western University, London ON N6A 5C1, Canada.
Gregory B GloorDepartment of Biochemistry, Schulich School of Medicine & Dentistry, Western University, London ON N6A 5C1, Canada.
David R EdgellDepartment of Biochemistry, Schulich School of Medicine & Dentistry, Western University, London ON N6A 5C1, Canada.ORCID 0000-0003-3858-6150

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Diatoms are globally significant microalgae that contribute ∼20% of oxygen production and exhibit remarkable metabolic diversity. The marine diatom

Indexed as

CRISPR-Cas SystemsDiatomsGenetic EngineeringGenomeGlycosylationPlasmidsCRISPR/Cas9Csy4fucosylationFucT multigene familyN-linked glycosylationPhaeodactylum tricornutumsgRNA array

Identifiers

PMID41979903
PMCPMC13185153

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.