ArticleJournal of extracellular vesicles2026
PREDICTOR: A Non-Enzymatic Catalytic Cascade Tool for in Situ Visualization of Small Extracellular Vesicle Surface glycoRNAs.
Article in Journal of extracellular vesicles, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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14 authors.
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Abstract
Glycosylated RNAs (glycoRNAs) are membrane-displayed RNA-glycan conjugates, but quantitative in situ analysis of low-abundance glycoRNAs on small extracellular vesicles (sEVs) remains challenging. Here, we develop PREDICTOR (proximity-encoded non-linear hybridization chain reaction circuit), an enzyme-free catalytic DNA cascade for imaging and quantifying surface glycoRNAs on intact sEVs. PREDICTOR uses a sialic acid aptamer and an RNA-sequence probe as split recognition modules, whose proximity on a single glycoRNA reconstitutes an initiator that drives dendritic DNA self-assembly and non-linear fluorescence amplification on the sEV surface. Across serial sEV inputs (5×10
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