ArticlePeerJ2026
DNA barcoding of North American freshwater copepods (Diaptomidae and Cyclopoida): an overview after 20 years with emphasis in the Mexican fauna, the transition between the Nearctic and Neotropics.
Article in PeerJ, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: In 2003, Paul Hebert proposed DNA barcoding, based on the first half of a standardized gene, the Cytochrome c Oxidase subunit I (COI), to identify animals. Subsequently, two large-scale projects enabled the sequencing of more than 1.3 million putative species worldwide. Two decades ago, we decided to adopt this approach as a tool to investigate the freshwater zooplankton diversity of Mexican aquatic systems. Several copepod species have been described by us with the aid of this marker, mainly of the family Diaptomidae, particularly of the species-rich genera Methods: In Mexico, we have sequenced the COI of 1,725 free-living freshwater copepods, including 925 diaptomid calanoids and 811 cyclopid cyclopoids, representing up to 43.7% of the total specimens sequenced for North America. To delineate the putative species diversity, we used the Barcode Index Number (BIN) and the Assemble Species by Automatic Partitioning method and compared both. For Results: Our results suggest that central-southeastern Mexico may represent a potential radiation center for speciose diaptomid genera like
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