An Oscillating-Flow Microfluidic PCR Method for Rapid and Flexible Detection of Periodontal Pathogens.

Zhenqing Li, Yueqing Wang, Jing Yang, Bo Yang, Yuan Zeng, Shinichi Sekine, Yoshinori Yamaguchi

Abstract read

In one paragraph

Article in Sensors (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Zhenqing LiEngineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China.ORCID 0000-0001-9060-7305

Yueqing WangEngineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China.

Jing YangFaculty of Engineering, Anhui Sanlian University, Hefei 230601, China.

Bo YangEngineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China.

Yuan ZengCollege of Medical Imaging, Shanghai University of Medicine & Health Sciences, Shanghai 201318, China.

Shinichi SekineDepartment of Preventive Dentistry, Graduate School of Dentistry, Osaka University, Osaka 565-0871, Japan.ORCID 0000-0003-4160-5578

Yoshinori YamaguchiComprehensive Research Organization, Waseda University, Tokyo 162-0041, Japan.

Funding

Anhui Sanlian University Research Team XJTD2025002

6 · The paper itself

Abstract

Polymerase chain reaction (PCR) is widely regarded as the gold standard for nucleic acid analysis; however, conventional thermal cycling limits its applicability in rapid and compact analytical systems. Here, we report an oscillating-flow microfluidic PCR method that enables rapid and flexible amplification by repeatedly shuttling the reaction mixture between two fixed-temperature zones. Unlike continuous-flow PCR, the proposed approach decouples PCR cycle number from microchannel geometry, allowing programmable cycling while reducing chip footprint. To enhance analytical reliability, polymer-assisted surface passivation using polyvinylpyrrolidone was employed to suppress nonspecific adsorption in polydimethylsiloxane (PDMS) microchannels, significantly improving amplification efficiency. Using

Indexed as

Microfluidic Analytical TechniquesMicrofluidicsPolymerase Chain ReactionPorphyromonas gingivalisTreponema denticolaHumansLab-On-A-Chip Devicesmicrofluidic chipperiodontal pathogenspolymerase chain reactionPorphyromonas gingivalisTreponema denticola

Identifiers

PMID41977911

DOI10.3390/s26072126

PMCPMC13075334

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.