Evidence map›Paper›PMID 41975067›Full record

ReviewNature protocols2026

Rapid volumetric bioprinting of pristine protein-based (bio)inks.

Maobin Xie, Liming Lian, Zhenrui Zhang, Zeng Lin, Emilio Mireles Guajardo, Jugal Kishore Sahoo, Guosheng Tang, Gang Li, Khoon S Lim, David L Kaplan and 1 more

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Nature protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Maobin Xie *The Fourth Affiliated Hospital of Guangzhou Medical University, School of Biomedical Engineering, Guangzhou Medical University, Guangzhou, P. R. China.ORCID http://orcid.org/0000-0001-9082-4510
Liming Lian *Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA.
Zhenrui ZhangDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA.ORCID http://orcid.org/0000-0003-0563-9148
Zeng LinDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA.
Emilio Mireles GuajardoDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA.
Jugal Kishore SahooDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.ORCID http://orcid.org/0000-0003-2503-9115
Guosheng TangDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA.ORCID http://orcid.org/0009-0009-2791-8448
Gang LiDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.ORCID http://orcid.org/0000-0002-8080-4134
Khoon S LimSchool of Medical Sciences, Charles Perkins Centre, The University of Sydney, Sydney, Australia.ORCID http://orcid.org/0000-0002-2486-196X
David L KaplanDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.ORCID http://orcid.org/0000-0002-9245-7774
Yu Shrike ZhangDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA. yszhang@bwh.harvard.edu.ORCID http://orcid.org/0000-0002-0045-0808

Funding

Tissue Engineering Resource Center: TTDP41EB027062 · NIBIB · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Gordana Vunjak-Novakovic · 2019 to 2026
$12.6M
High-throughput Imaging-integrated Vascular Model for Understanding Thromboembolism and Therapeutics ScreeningR01HL166522 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI Junjie Yao, Y. Shrike Zhang · 2023 to 2026
$2.7M
A Bioprinted Volumetric Model of Vascularized GlioblastomaR01CA282451 · NCI · BRIGHAM AND WOMEN'S HOSPITAL · PI Kaisorn Lee Chaichana, Y. Shrike Zhang · 2023 to 2026
$1.7M
Intra-Articular Delivery of Sustained Release NF-kB Antagonists In ArthritisR01AR070975 · NIAMS · WASHINGTON UNIVERSITY · PI SETTON, LORI A. · 2017 to 2021
$1.7M
Foundation for the National Institutes of Health (Foundation for the National Institutes of Health, Inc.) P41EB027062National Science Foundation (NSF) CBET-EBMS-1936105NCI NIH HHS R01 CA282451NHLBI NIH HHS R01 HL166522NIAMS NIH HHS R01 AR070975NIBIB NIH HHS P41 EB027062
6 · The paper itself

Abstract

Volumetric bioprinting (VBP) enables the rapid photopolymerization of 3D constructs by modifying the illumination patterns within a build volume. However, only a few unmodified, pristine protein-based bioinks can be used for VBP, making the resulting (bio)printed volumes sometimes incompatible with further modification steps required for extended applications and thus limiting the wider adoption of VBP. We have recently developed new methods for VBP, in which unmodified protein-based (bio)inks with tyrosine groups, including those based on silk, decellularized extracellular matrix (dECM) and gelatin, can be (bio)printed, in their pristine state, by using the tris(2,2-bipyridyl)dichlororuthenium(II) hexahydrate/sodium persulfate photoinitiator system to form sophisticated shapes and architectures. Here, we provide step-by-step instructions to complete the VBP process and include the characterization of these bioinks. After treatment, the volumetrically printed silk sericin constructs show properties including reversible shrinkage and expansion, or shape-memory, whereas the volumetrically printed silk fibroin constructs exhibit broadly tunable mechanical performances ranging from a few hundred pascals to hundreds of megapascals. Both types of silk-based (bio)inks as well as dECM (bio)inks are cytocompatible. We further cover several demonstrations that show the potential uses of volumetrically (bio)printed silk and dECM constructs in clinical and biomedical applications.

Identifiers

PMID41975067
PMCPMC13380836

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.