Evidence map›Paper›PMID 41974831›Full record

ArticleNature chemistry2026

tRNA-deacylase-directed discovery of biosynthetic pathways.

Douglas C Millar, Yu Zhou, Jorge A Marchand, Helena Roberts-Mataric, Tavis Chen, Calvin X Hu, Jason Fang, Shannon Millar, Elizabeth A Stone, Jeffrey G Pelton and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Douglas C MillarDepartment of Chemical and Biomolecular Engineering, University of California at Berkeley, Berkeley, CA, USA.ORCID 0009-0003-2902-0312
Yu ZhouDepartment of Chemistry, Princeton University, Princeton, NJ, USA.ORCID 0000-0002-1882-9891
Jorge A MarchandDepartment of Chemical and Biomolecular Engineering, University of California at Berkeley, Berkeley, CA, USA.ORCID 0000-0002-7765-610X
Helena Roberts-MataricDepartment of Chemistry, University of California at Berkeley, Berkeley, CA, USA.ORCID 0009-0001-4047-0483
Tavis ChenDepartment of Chemical and Biomolecular Engineering, University of California at Berkeley, Berkeley, CA, USA.ORCID 0009-0008-2102-9036
Calvin X HuDepartment of Chemistry, University of California at Berkeley, Berkeley, CA, USA.
Jason FangDepartment of Chemistry, University of California at Berkeley, Berkeley, CA, USA.ORCID 0000-0003-3382-9539
Shannon MillarDepartment of Industrial Engineering and Operations Research, University of California at Berkeley, Berkeley, CA, USA.
Elizabeth A StoneDepartment of Chemistry, University of California at Berkeley, Berkeley, CA, USA.ORCID 0000-0002-2253-3452
Jeffrey G PeltonQB3 Institute, University of California at Berkeley, Berkeley, CA, USA.
Max B SosaDepartment of Chemistry, University of California at Berkeley, Berkeley, CA, USA.
Michelle C Y ChangDepartment of Chemical and Biomolecular Engineering, University of California at Berkeley, Berkeley, CA, USA. mcchang@princeton.edu.ORCID 0000-0003-3747-7630

Funding

Univ of Calif Berkeley Chemistry-Biology Interface ProgramT32GM066698 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI CHANG, CHRISTOPHER J., FRANCIS, MATTHEW B · 2004 to 2023
$7.5M
Proposal for Central California 900 MHZ NMR SpectrometerP41GM068933 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI WEMMER, DAVID E · 2003 to 2007
$5.9M
Acquisition of a Cryoprobe and Related Accessories for a 600 MHz NMR Spectrometer to Support Health Research Activities at the University of California, BerkeleyS10OD024998 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI HARTWIG, JOHN F · 2018 to 2018
$265k
Howard Hughes Medical Institute (HHMI) Gilliam FellowshipJane Coffin Childs Memorial Fund for Medical Research (JCC Fund) Postdoctoral FellowshipNational Science Foundation (NSF) 2204014National Science Foundation (NSF) GRFPNIGMS NIH HHS P41 GM068933NIGMS NIH HHS T32 GM066698NIH HHS S10 OD024998U.S. Department of Health & Human Services | National Institutes of Health (NIH) 1 T32 GM066698
6 · The paper itself

Abstract

Amino acids are one of nature's most privileged building blocks for generating molecular diversity on length scales ranging from small molecules to proteins. While amino acid products arising from certain class-defined biosynthetic pathways can be found with established bioinformatic strategies, those that fall outside of these types remain difficult to identify. Here, to address this challenge, we have developed an approach to find biosynthetic gene clusters (BGCs) that utilize and modify amino acid monomers while remaining agnostic to biosynthetic class. We demonstrate that tRNA deacylases specific for host-synthesized non-canonical amino acids (ncAAs) serve as a common genomic marker of ncAA metabolism. Using this approach, we show that thousands of cryptic BGCs can be identified and demonstrate the discovery of BGCs for several distinct ncAAs as well as a hydrazide-containing tripeptide. We anticipate this approach will have broad applications for discovering natural products with ncAAs and beyond.

Indexed as

Amino AcidsAminoacyltransferasesBiosynthetic PathwaysRNA, TransferMultigene FamilyAmino AcidsAminoacyltransferasesRNA, Transfer

Identifiers

PMID41974831
PMCPMC13149037

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.