Evidence map›Paper›PMID 41974741›Full record

ArticleNature communications2026

Double-helix optical point spread function enables real-time mesoscopic 3D functional microangiography in the living mouse brain and skull.

Baoyuan Zhang, Shiyao Guo, Lin Tang, Yi Chen, Lukas Glandorf, Etienne Jessen, Xuyang Chang, Tian Jin, Michael Reiss, Shuxin Lyu and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Baoyuan ZhangInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.
Shiyao GuoSchool of Physics Science and Engineering, Tongji University, Shanghai, China.
Lin TangInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.ORCID http://orcid.org/0009-0008-3399-1134
Yi ChenInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.ORCID http://orcid.org/0000-0003-3980-9184
Lukas GlandorfInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.ORCID http://orcid.org/0000-0003-4968-2906
Etienne JessenInstitute for Mechanics, Computational Mechanics Group, Technical University of Darmstadt, Darmstadt, Germany.ORCID http://orcid.org/0000-0003-0276-6029
Xuyang ChangInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.ORCID http://orcid.org/0000-0001-5696-0033
Tian JinInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.
Michael ReissInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.ORCID http://orcid.org/0000-0003-3583-3451
Shuxin LyuInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.
Qiang FuKing Abdullah University of Science and Technology, Thuwal, Saudi Arabia.ORCID http://orcid.org/0000-0001-6395-8521
Hadi AmataKing Abdullah University of Science and Technology, Thuwal, Saudi Arabia.ORCID http://orcid.org/0000-0002-9343-729X
Wolfgang HeidrichKing Abdullah University of Science and Technology, Thuwal, Saudi Arabia.ORCID http://orcid.org/0000-0002-4227-8508
Chaim GlückInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.ORCID http://orcid.org/0000-0002-8754-9965
Dominik SchillingerInstitute for Mechanics, Computational Mechanics Group, Technical University of Darmstadt, Darmstadt, Germany.
Bruno WeberInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.ORCID http://orcid.org/0000-0002-9089-0689
Xosé Luís Deán-BenInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.ORCID http://orcid.org/0000-0002-8557-7778
Weibo WangCenter of Ultra-Precision Optoelectronic Instrument Engineering, Harbin Institute of Technology, Harbin, China. wwbhit@hit.edu.cn.
Xiong DunSchool of Physics Science and Engineering, Tongji University, Shanghai, China. dunx@tongji.edu.cn.
Daniel RazanskyInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland. daniel.razansky@uzh.ch.ORCID http://orcid.org/0000-0001-8676-0964
Zhenyue ChenSchool of Physics Science and Engineering, Tongji University, Shanghai, China. zhenyue_chen@tongji.edu.cn.ORCID http://orcid.org/0000-0001-9508-7521
Quanyu ZhouInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland. quanyu.zhou@uzh.ch.ORCID http://orcid.org/0000-0001-7719-4649

Funding

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation) 10.003.762Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation) 10.006.824
6 · The paper itself

Abstract

Quantitative, volumetric imaging of cerebrovascular networks and microcirculation is essential for understanding brain function. However, rapid mesoscopic 3D imaging remains challenging because of fundamental trade-offs between spatiotemporal resolution, field of view, and sensitivity to functional parameters. Here we present a mesoscopic fluorescence imaging platform featuring a double-helix phase mask for real-time, depth-resolved measurements through the intact mouse skull. The compact phase-mask design is compatible with both laser-scanning and widefield microscopy. Using multifocal laser scanning, we demonstrate real-time volumetric in vivo imaging while discriminating calvarial from cerebral vasculature across 6.6×6.6×0.8 mm

Indexed as

AngiographyBrainImaging, Three-DimensionalSkullAnimalsCerebrovascular CirculationGliomaMaleMiceMicrocirculationMicroscopy, ConfocalOptical Imaging

Identifiers

PMID41974741
PMCPMC13249855

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.